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Journal of Plant Growth Regulation

, Volume 29, Issue 3, pp 289–296 | Cite as

Cytokinin Primes Plant Responses to Wounding and Reduces Insect Performance

  • Christopher Dervinis
  • Christopher J. Frost
  • Susan D. Lawrence
  • Nicole G. Novak
  • John M. Davis
Article

Abstract

We report a potential role of endogenous cytokinin supply in priming plant defense against herbivory. Cytokinin priming significantly reduced weight gain by insect larvae. Unlike previously described priming by volatile compounds, priming by cytokinin did not overcome vascular restrictions on systemic wound signaling. However, similar to priming by volatile compounds, cytokinin priming occurred upstream of accumulation of jasmonic acid and its precursor linolenic acid in mechanically wounded source leaves. Cytokinin priming significantly altered wound-induced accumulation of transcripts encoding homologs of allene oxide synthase, trypsin inhibitor, and chitinase. Cytokinin priming may reflect coordination between aboveground inducible defense against herbivory and belowground processes such as nutrient availability. These findings should encourage investigations of how genetic alterations in cytokinin signaling and response pathways may affect plant–herbivore interactions.

Keywords

Cytokinin Priming Wounding Populus 

Notes

Acknowledgments

This research was supported by grants from the Department of Energy, Office of Science, Office of Biological and Environmental Research (DE-AC05-00OR22725 to JMD), and the United States Department of Agriculture (USDA-NRI 2007-35302-18087 to CJF).

Supplementary material

344_2009_9135_MOESM1_ESM.doc (32 kb)
Supplementary material 1 (DOC 32 kb)
344_2009_9135_MOESM2_ESM.tif (1.5 mb)
Supplementary material 2 (TIFF 1510 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Christopher Dervinis
    • 1
  • Christopher J. Frost
    • 2
  • Susan D. Lawrence
    • 3
  • Nicole G. Novak
    • 3
  • John M. Davis
    • 1
  1. 1.School of Forest Resources and ConservationUniversity of FloridaGainesvilleUSA
  2. 2.School of Forest ResourcesPennsylvania State UniversityUniversity ParkUSA
  3. 3.U.S. Department of Agriculture—Agricultural Research ServiceInvasive Insect Biocontrol and Behavior LaboratoryBeltsvilleUSA

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