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Journal of Plant Growth Regulation

, Volume 25, Issue 1, pp 10–17 | Cite as

Exogenous Catalase and Ascorbate Modify the Effects of Abscisic Acid (ABA) on Root Hydraulic Properties in Phaseolus vulgaris L. Plants

  • Ricardo ArocaEmail author
Article

Abstract

Abscisic acid (ABA) modifies the hydraulic properties of roots by increasing root water flux (Jv). The role of reactive oxygen species (ROS) in this ABA-induced process was evaluated. At the same time, some antioxidant enzyme activities in root tissues were measured. Phaseolus vulgaris plants were grown hydroponically, and different concentrations of ABA in combination with catalase enzyme or ascorbate were added to the nutrient solution. Catalase treatment had no effect by itself (no ABA) and had little or only a small stimulatory effect at ABA concentrations of 1, 50, and 100 μM, but it partially inhibited the ABA effect at 5 μM. Ascorbate by itself doubled Jv and root hydraulic conductance over the control value. In the presence of ABA, ascorbate partially or, at 100 μM, completely inhibited that ABA stimulation of Jv. These results are discussed in relationship to the possibility that ABA signaling in the roots involves ROS.

Keywords

Abscisic acid Ascorbate Catalase Phaseolus vulagaris Reactive oxygen species Root water transport 

Notes

Acknowledgments

The author thanks Prof. M. J. Chrispeels (University of California San Diego) for making his laboratory facilities available for these experiments, as well as for stimulating discussions and for editing the manuscript. The author was supported by a postdoctoral fellowship from the Ministerio de Educación y Ciencia (Spain). The author is also grateful to Dr. M. J. Martín for help during the experiments.

References

  1. Aebi H. 1984. Catalase in vitro. Methods Enzymol 105:121–126PubMedGoogle Scholar
  2. Aroca R, Amodeo G, Fernández-Illescas S, Herman EM, Chaumont F, et al. 2005. The role of aquaporins and membrane damage in chilling and hydrogen peroxide induce changes in the hydraulic conductance of maize roots. Plant Physiol 137:341–353CrossRefPubMedGoogle Scholar
  3. Aroca R, Irigoyen JJ, Sánchez-Díaz M. 2001. Photosynthetic characteristics and protective mechanisms against oxidative stress during chilling and subsequent recovery in two maize varieties differing in chilling sensitivity. Plant Sci 161:719–726CrossRefGoogle Scholar
  4. Aroca R, Vernieri P, Irigoyen JJ, Sánchez-Díaz M, Tognoni F, et al. 2003. Involvement of abscisic acid in leaf and root of maize (Zea mays L.) in avoiding chilling-induced water stress. Plant Sci 165:671–679CrossRefGoogle Scholar
  5. BassiriRad H, Radin JW. 1992. Temperature-dependent water and ion transport properties of barley and sorghum roots. Plant Physiol 99:34–37Google Scholar
  6. Chinnusany V, Schumaker K, Zhu J-K. 2004. Molecular genetics perspectives on crosstalk and specificity in abiotic stress signalling in plants. J Exp Bot 55:225–236Google Scholar
  7. Collins JC, Morgan M. 1980. The influence of temperature on the abscisic acid stimulated water flow from excised maize roots. New Phytol 84:19–26Google Scholar
  8. Cross JB, Currier RP, Torraco DJ, Vanderberg LA, Wagner GL, et al. 2003. Killing of Bacillus spores by aqueous dissolved oxygen, ascorbic acid, and copper ions. Appl Environ Microbiol 69:2245–2252CrossRefPubMedGoogle Scholar
  9. Desikan R, Cheung M-K, Bright J, Henson D, Hancock JT, et al. 2004. ABA, hydrogen peroxide and nitric oxide signalling in stomatal guard cells. J Exp Bot 55:205–212PubMedGoogle Scholar
  10. Fan S, Blake TJ. 1994. Abscisic acid induced electrolyte leakage in woody species with contrasting ecological requirements. Physiol Plant 90:414–419Google Scholar
  11. Fernández-García N, Martínez V, Cerdá A, Carvajal M. 2002. Water and nutrient uptake of grafted tomato plants grown under saline conditions. J Plant Physiol 159:899–905Google Scholar
  12. Foyer CH, Noctor G. 2005. Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in physiological context. Plant Cell Environ 28:1056–1071CrossRefGoogle Scholar
  13. Henzler T, Waterhouse RN, Smith AJ, Carvajal M, Cooke DT, et al. 1999. Diurnal variations in hydraulic conductivity and root pressure can be correlated with the expression of putative aquaporins in the roots of Lotus japonicus. Planta 210:50–60CrossRefPubMedGoogle Scholar
  14. Henzler T, Ye Q, Steudle E. 2004. Oxidative gating of water channels (aquaporins) in Chara by hydroxyl radicals. Plant Cell Environ 27:1184–1195CrossRefGoogle Scholar
  15. Hose E, Steudle E, Hartung W. 2000. Abscisic acid and hydraulic conductivity of maize roots: a study using cell- and root-pressure probes. Planta 211:874–882CrossRefPubMedGoogle Scholar
  16. Jiang M, Zhang J. 2001. Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol 42:1265–1273CrossRefPubMedGoogle Scholar
  17. Jiang M, Zhang J. 2002. Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings. Planta 215:1022–1030CrossRefPubMedGoogle Scholar
  18. Köhler B, Hills A, Blatt MR. 2003. Control of guard cell ion channels by hydrogen peroxide and abscisic acid indicates their action through alternate signaling pathways. Plant Physiol 131:385–388PubMedGoogle Scholar
  19. Ktitorova IN, Skobeleva OV, Sharova EI, Ermakov EL. 2002. Hydrogen peroxide appears to mediate a decrease in hydraulic conductivity in wheat roots under salt stress [in Russian]. J Plant Physiol 49:369–380Google Scholar
  20. Lee SH, Singh AP, Chung GC. 2004. Rapid accumulation of hydrogen peroxide in cucumber roots due to exposure to low temperature appears to mediate decrease in water transport. J Exp Bot 55:1733–1741CrossRefPubMedGoogle Scholar
  21. Lin CC, Kao CH. 2001. Abscisic acid induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. Plant Sci 160:323–329CrossRefPubMedGoogle Scholar
  22. Ludewig M, Dörffling K, Seifert H. 1988. Abscisic acid and water transport in sunflowers. Planta 175:325–333CrossRefGoogle Scholar
  23. Luu DT, Maurel C. 2005. Aquaporins in a challenging environment: molecular gears for adjusting plant water status. Plant Cell Environ 28:85–96CrossRefGoogle Scholar
  24. Markhart AH III, Fiscus EL, Naylor AW, Kramer PJ. 1979. Effect of abscisic acid on root hydraulic conductivity. Plant Physiol 64:611–614Google Scholar
  25. Mittler R, Vanderauwera S, Gollery M, Van Breusegem F. 2004. Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498CrossRefPubMedGoogle Scholar
  26. Mori IC, Schroeder JI. 2004. Reactive oxygen species activation of plant Ca2+ channels. A signaling mechanism in polar growth, hormone transduction, stress signaling, and hypothetical mechanotransduction. Plant Physiol 135:702–708CrossRefPubMedGoogle Scholar
  27. Noctor G, Foyer CH. 1998. Ascorbate and glutathione: keeping active oxygen species under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279CrossRefPubMedGoogle Scholar
  28. Pignocchi C, Foyer CH. 2003. Apoplastic ascorbate metabolism and its role in the regulation of cell signalling. Curr Opin Plant Biol 6:379–389CrossRefPubMedGoogle Scholar
  29. Schraut D, Heilmeier H, Hartung W. 2005. Radial transport of water and abscisic acid (ABA) in roots of Zea mays under conditions of nutrient deficiency. J Exp Bot 56:879–886CrossRefPubMedGoogle Scholar
  30. Tomos AD, Leigh RA. 1999. The pressure probe: a versatile tool in plant cell physiology. Annu Rev Plant Physiol Plant Mol Biol 50:447–472CrossRefPubMedGoogle Scholar
  31. Tsai Y-C, Kao CH. 2004. The involvement of hydrogen peroxide in abscisic acid-induced activities of ascorbate peroxidase and glutathione reductase in rice roots. J Plant Growth Regul 43:207–212CrossRefGoogle Scholar
  32. Van Breusegem F, Vranová E, Dat JF, Inzé D. 2001. The role of active oxygen species in plant signal transduction. Plant Sci 161:405–414Google Scholar
  33. Vysotskaya LB, Arkhipova TN, Timergalina LN, Dedov AV, Veselov SY, et al. 2004. Effect of partial root excision on transpiration, root hydraulic conductance and leaf growth in wheat seedlings. Plant Physiol Biochem 42:251–255CrossRefPubMedGoogle Scholar
  34. Wan X, Zwiazek JJ. 2001. Root water flow and leaf stomatal conductance in aspen (Populus tremuloides) seedlings treated with abscisic acid. Planta 213:741–747CrossRefPubMedGoogle Scholar
  35. Zhang J, Zhang X, Liang J. 1995. Exudation rate and hydraulic conductivity of maize roots are enhanced by soil drying and abscisic acid treatment. New Phytol 131:329–336Google Scholar
  36. Zhang X, Zhang L, Dong F, Gao J, Galbraith DW, et al. 2001. Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba. Plant Physiol 126:1438–1448CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Division of Biological SciencesUniversity of California San DiegoLa JollaUSA

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