Journal of Plant Growth Regulation

, Volume 25, Issue 1, pp 79–88 | Cite as

Regenerative Capacity of Cacti Schlumbergera and Rhipsalidopsis in Relation to Endogenous Phytohormones, Cytokinin Oxidase/Dehydrogenase, and Peroxidase Activities

  • Sridevy Sriskandarajah
  • Els Prinsen
  • Václav Motyka
  • Petre Ivanov Dobrev
  • Margrethe Serek
Article

Abstract

The recalcitrant nature and increased regenerative capacity in relation to in vitro subcultures in two cactus genera Rhipsalidopsis (Easter cactus) and Schlumbergera (Christmas cactus) were studied by examining the endogenous concentrations of several endogenous phytohormones and enzyme activities. Leaf tissue from greenhouse-grown mother plants, in vitro subcultures 1 and 3, and callus tissues were analyzed and correlated with regenerative ability. The cytokinins present in the two cacti genera were mainly isopentenyl-type derivatives. The total content of isopentenyl-type cytokinins in greenhouse-grown leaves of Rhipsalidopsis was more than twice the amount found in greenhouse-grown leaves of Schlumbergera. The total cytokinin content decreased during subculturing. Cytokinin oxidase/dehydrogenase (CKX, EC 1.4.3.18/1.5.99.12) activity increased during subculturing. In Schlumbergera there is no effect of subculturing on CKX and related cytokinin homeostasis. The total peroxidase (POX, EC 1.11.1.7) activity in greenhouse-grown leaves of both genera was low, and the activity increased significantly during subculturing, more specifically in the tissue of Rhipsalidopsis. The results clearly indicated that an enhanced auxin metabolism (biosynthesis, conjugation/deconjugation, and POX activity), in combination with an enhanced CKX activity, shifts the auxin and cytokinin pool, favoring adventitious shoot formation in Rhipsalidopsis, whereas the low level of POX activity, together with auxin autotrophy/conjugation, makes Schlumbergera more recalcitrant.

Keywords

Cytokinin dehydrogenase Cytokinin oxidase Endogenous phytohormones In vitro regeneration Peroxidase Rhipsalidopsis Schlumbergera 

Notes

Acknowledgments

A part of the project performed by S.S. and M.S. was funded by the Danish Ministry of Agriculture and Fisheries (93s-2466-Å01-01430) and by the Danish Schlumbergera Growers and Breeders Gartneriet Thoruplund A/S (Odense), Rohdes Gartneri A/S (Kerteminde), Gartneriet PKM ApS (Odense), and Hansson DK (Søndersø). The work of P.I.D. and V.M. was supported by the Grant Agency of the Czech Republic (grant 206/03/0313) and that done by E.P. was supported by the Scientific Research Network of the Fund for Scientific Research Flanders (Belgium) (WO.038.04N). The authors thank Prof. Emeritus Errol W. Hewett (Massey University, Palmerston North, New Zealand) for linguistic editing of the manuscript. They also thank Sevgi Öden and Marie Korecká for their skillful technical assistance.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Sridevy Sriskandarajah
    • 1
  • Els Prinsen
    • 2
  • Václav Motyka
    • 3
  • Petre Ivanov Dobrev
    • 3
  • Margrethe Serek
    • 1
    • 4
  1. 1.Department of Agricultural SciencesCrop Science, Floriculture, The Royal Veterinary and Agricultural UniversityDenmark
  2. 2.Department of BiologyUniversity of AntwerpBelgium
  3. 3.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicCzech Republic
  4. 4.Ornamental and Woody Plant Science, Department of Natural SciencesUniversity of HanoverGermany

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