Advertisement

Journal of Plant Growth Regulation

, Volume 22, Issue 3, pp 205–216 | Cite as

Effects of Benzyladenine and Naphthalene Acetic Acid on Growth and Camptothecin Accumulation in Camptotheca acuminata Seedlings

  • Zhanhai LiEmail author
  • Zhijun Liu
Article

Abstract

The effects of the cytokinin benzyladenine (BA) and the auxin naphthalene acetic acid (NAA) on Camptotheca acuminata Decaisne growth and camptothecin (CPT) accumulation (leaf CPT concentration and total leaf CPT yield) were studied in a hydroponic culture system for three weeks. Increasing BA concentrations from 0 to 3 mg l−1 in growth medium decreased plant height, stem weight, and leaf weight but increased root weight. High BA levels (1 and 3 mg l−1) increased leaf CPT concentration (% of dry weight), whereas BA applications had no effect on total leaf CPT yield, the product of leaf CPT concentration and total leaf dry weight per seedling. There was a positive correlation between root weight and leaf CPT concentration under BA treatments. NAA supplementations (from 0.5 to 4 mg l−1) to growth medium reduced plant height, leaf number, leaf length, specific leaf weight, plant weight, stem weight, and leaf weight compared with the NAA control. Meanwhile, there were no differences in plant height, leaf length, and specific leaf weight among the NAA supplementations. NAA applications had no effect on leaf CPT concentration and NAA applications decreased total leaf CPT yield. There were negative correlations between leaf number and leaf CPT concentration, leaf length and leaf CPT concentration under NAA treatments. Our results suggest that BA applications from 0.3 to 3 mg l−1 are not helpful for achieving high total leaf CPT yield and NAA applications from 0.5 to 4 mg l−1 decrease total leaf CPT yield.

Keywords

Alkaloid Anti-cancer plant Auxin BA Camptotheca acuminata Decaisne (family Nyssaceae) Camptothecin Cytokinin NAA 

Notes

Acknowledgements

The authors thank Drs. Charles E. Johnson, Seth J. Johnson, Michael Stine, and Martin A. Hjortso for reviewing early drafts of the manuscript. Thanks also go to Ying Yu for a large amount of CPT analysis, and student workers Stefanie Becnel and Bradley Leblanc for their assistance in conducting the experiments. This research was funded by grants from the Louisiana Board of Regents Research Competitiveness Subprogram (LEQSF(1998-01)-RD-A-02), the Pacific West Cancer Fund in Seattle, Washington, USA, and McIntire-Stennis Cooperative Forestry Research Fund (LAB93381).

References

  1. 1.
    Arvy, MP, Imbault, N, Naudascher, F, Thiersault, M, Doireau, P 19942,4-D and alkaloid accumulation in periwinkle cell suspensions.Biochimie-Paris76410416CrossRefGoogle Scholar
  2. 2.
    Carpin, S, Ouelhazi, L, Filali, M, Chenieux, JC, Rideau, M, Hamdi, S 1997The relation between the accumulation of a 28 kDa polypeptide and that of indole alkaloids in Catharanthus roseus cell suspension cultures.J Plant Physiol150452457Google Scholar
  3. 3.
    Couillerot, E, Caron, C, Audran, JC, Jardillier, JC, Chenieux, JC 1996Furoquinoline alkaloid accumulation in Fagara zanthoxyloides cell cultures is highly dependent on the presence of exogenous benzylaminopurine.Plant Growth Reg19203206Google Scholar
  4. 4.
    Decendit, A, Liu, D, Ouelhazi, L, Doireau, P, Merillon, JM, Rideau, M 1992Cytokinin-enhanced accumulation of indole alkaloids in Catharanthus roseus cell cultures: the factors affecting the cytokinin response.Plant Cell Rep11400403Google Scholar
  5. 5.
    El Bahr, MK, Hussein, MS, Moursy, HA 1989Effect of some growth regulators on the growth and alkaloid production of Datura stramonium L. cultured in vitro.Egyptian J Bot325362Google Scholar
  6. 6.
    Gantet, P, Imbault, N, Thiersault, M, Doireau, P 1997Inhibition of alkaloid accumulation by 2,4-D in Catharanthus roseus cell suspension is overcome by methyl jasmonate.Acta Botanica Gallica144501508Google Scholar
  7. 7.
    Goddijn, OJM, De Kam, RJ, Zanetti, A, Schileperoort, RA, Hoge, JHC 1992Auxin rapidly down-regulated transcription of the tryptophan decarboxylase gene from Catharanthus roseus.Plant Mol Biol1811131120PubMedGoogle Scholar
  8. 8.
    Khanam, N, Khoo, CR, Khan, AG 2000Organogenesis, differentiation and histolocalization of alkaloids in cultured tissues and organs of Duboisia myoporoides R.Ann Bot86745752CrossRefGoogle Scholar
  9. 9.
    Kjeldsen, E, Svejstrup, JQ, Gromova, II, Alsner, J, Westergaard, O 1992Camptothecin inhibits both the cleavage and religation of eukaryotic DNA topoisomerase I.J Mol Biol22810251030Google Scholar
  10. 10.
    Liu, Z 2000Drought-induced in vivo synthesis of camptothecin in Camptotheca acuminata seedlings.Physiol Plant110483488CrossRefGoogle Scholar
  11. 11.
    Liu, Z, Adams, JC 1996Camptothecin yield and distribution within Camptotheca acuminata trees cultivated in Louisiana.Can J Bot74360365Google Scholar
  12. 12.
    Liu, Z, Adams, JC, Viator, HP, Constantin, RJ, Carpenter, SB 1999Influence of soil fertilization, plant spacing, and coppicing on growth, stomatal conductance, abscisic acid and camptothecin levels in Camptotheca acuminata seedlings.Physiol Plant105402408CrossRefGoogle Scholar
  13. 13.
    Liu, Z, Carpenter, SB, Bourgeois, WJ, Yu, Y, Constantin, RJ, Falcon, MJ, Adams, JC 1998Variation in the secondary metabolite camptothecin in relation to tissue age and season in Camptotheca acuminata.Tree Physiol18265270PubMedGoogle Scholar
  14. 14.
    Liu, Z, Carpenter, SB, Constantin, RJ 1997Camptothecin production in Camptotheca acuminata seedlings in response to shading and flooding.Can J Bot75368373Google Scholar
  15. 15.
    Liu, Z, Li, Z 2001Micropropagation of Camptotheca acuminata Decaisne from axillary buds, shoot tips and seed embryo in a tissue culture system.In Vitro Cell Dev Biol378488CrossRefGoogle Scholar
  16. 16.
    Lopez-Meyer, M, Nessler, CL 1997Tryptophan decarboxylase is encoded by two autonomously regulated genes in Camptotheca acuminata which are differentially expressed during development and stress.Plant J1111671175CrossRefGoogle Scholar
  17. 17.
    Lopez-Meyer, M, Nessler, CL, McKnight, TD 1994Sites of accumulation of the anti-tumor alkaloid camptothecin in Camptotheca acuminata.Planta Med60558560PubMedGoogle Scholar
  18. 18.
    Ouelhazi, L, Filali, M, Decendit, A, Chenieux, JC, Rideau, M 1993Differential protein accumulation in zeatin- and 2,4-D-treated cells of Catharanthus roseus: correlation with indole alkaloid biosynthesis.Plant Physiol Biochem31421431Google Scholar
  19. 19.
    Ouelhazi, L, Hamdi, S, Chenieux, JC, Rideau, M 1994Cytokinin and auxin-induced regulation of protein synthesis and poly (A)+RNA accumulation in Catharanthus roseus cell cultures.J Plant Physiol144167174Google Scholar
  20. 20.
    Pasquali, G, Goddijn, OJM, De Waal, A, Verpoorte, R, Schilperoort, RA, Hoge, JHC, Memelink, J 1992Coordinated regulation of two indole alkaloid biosynthetic genes from Catharanthus roseus by auxin and elicitors.Plant Mol Biol1811211131PubMedGoogle Scholar
  21. 21.
    Siah, CL, Doran, PM 1991Enhanced codeine and morphine production in suspended Papaver somniferum cultures after removal of exogenous growth regulators.Plant Cell Rep10349353Google Scholar
  22. 22.
    Song, SH, Byun, SY 1998Characterization of cell growth and CPT production in cell cultures of Camptotheca acuminata.J Microbiol Biotechnol8631638Google Scholar
  23. 23.
    Taiz, L, Zeiger, E 1998Plant physiology, 3rd ed.Sinauer AssociatesSunderland543Google Scholar
  24. 24.
    Vanhala, L, Eava, M, Lapinjoki, S, Hiltunen, R, Oksman, CKM 1998Effect of growth regulators on transformed root cultures of Hyoscyamus muticus.J Plant Physiol153475481Google Scholar
  25. 25.
    Wall, ME, Wani, MC, Cook, CE, Palmer, KH 1966Plant anti-tumor agents I. The isolation and structure of camptothecin -a novel alkaloidal leukemia and tumor inhibitor from Camptotheca acuminata.J Am Chem Soc8838883890Google Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.School of Renewable Natural ResourcesLouisiana State University Agricultural Center, Baton Rouge, LA 70803USA
  2. 2.Department of Biostatistics and Medical Informatics, University of Wisconsin-Madison, Madison, WI 53792USA

Personalised recommendations