Journal of Plant Growth Regulation

, Volume 22, Issue 3, pp 205–216 | Cite as

Effects of Benzyladenine and Naphthalene Acetic Acid on Growth and Camptothecin Accumulation in Camptotheca acuminata Seedlings

  • Zhanhai LiEmail author
  • Zhijun Liu


The effects of the cytokinin benzyladenine (BA) and the auxin naphthalene acetic acid (NAA) on Camptotheca acuminata Decaisne growth and camptothecin (CPT) accumulation (leaf CPT concentration and total leaf CPT yield) were studied in a hydroponic culture system for three weeks. Increasing BA concentrations from 0 to 3 mg l−1 in growth medium decreased plant height, stem weight, and leaf weight but increased root weight. High BA levels (1 and 3 mg l−1) increased leaf CPT concentration (% of dry weight), whereas BA applications had no effect on total leaf CPT yield, the product of leaf CPT concentration and total leaf dry weight per seedling. There was a positive correlation between root weight and leaf CPT concentration under BA treatments. NAA supplementations (from 0.5 to 4 mg l−1) to growth medium reduced plant height, leaf number, leaf length, specific leaf weight, plant weight, stem weight, and leaf weight compared with the NAA control. Meanwhile, there were no differences in plant height, leaf length, and specific leaf weight among the NAA supplementations. NAA applications had no effect on leaf CPT concentration and NAA applications decreased total leaf CPT yield. There were negative correlations between leaf number and leaf CPT concentration, leaf length and leaf CPT concentration under NAA treatments. Our results suggest that BA applications from 0.3 to 3 mg l−1 are not helpful for achieving high total leaf CPT yield and NAA applications from 0.5 to 4 mg l−1 decrease total leaf CPT yield.


Alkaloid Anti-cancer plant Auxin BA Camptotheca acuminata Decaisne (family Nyssaceae) Camptothecin Cytokinin NAA 



The authors thank Drs. Charles E. Johnson, Seth J. Johnson, Michael Stine, and Martin A. Hjortso for reviewing early drafts of the manuscript. Thanks also go to Ying Yu for a large amount of CPT analysis, and student workers Stefanie Becnel and Bradley Leblanc for their assistance in conducting the experiments. This research was funded by grants from the Louisiana Board of Regents Research Competitiveness Subprogram (LEQSF(1998-01)-RD-A-02), the Pacific West Cancer Fund in Seattle, Washington, USA, and McIntire-Stennis Cooperative Forestry Research Fund (LAB93381).


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.School of Renewable Natural ResourcesLouisiana State University Agricultural Center, Baton Rouge, LA 70803USA
  2. 2.Department of Biostatistics and Medical Informatics, University of Wisconsin-Madison, Madison, WI 53792USA

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