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Expression profiles of sex–related genes in gonads of genetic male Takifugu rubripes after 17β–estradiol immersion

  • Peng Hu
  • Bin Liu
  • Qian Ma
  • Shufang Liu
  • Xinfu LiuEmail author
  • Zhimeng Zhuang
Article

Abstract

Estradiol treatment during early life stages of tiger puffer Takifugu rubripes induces feminization in genetic males. However, the ovaries in genetic males may revert to testes once estradiol treatment is halted. Therefore studies should investigate molecular mechanisms underlying ovary–to–testis recovery in genetic males after treatment. In the present study, tiger puffer were exposed to 10, and 100 μg/L 17β–estradiol (E2 ) from 15 to 100 days post–hatching (dph), then gonad phenotypes and expression profiles of six sex–related genes (cyp19a, foxl2, dmrt1, amh, sox9a, and sox9b) were characterized after the exposure. Results showed that both 10 and 100 μg/L E2 induced ovarian development in genetic males at 100 dph. However, all ovaries induced by 10 μg/L E2 first developed into intersexual gonads and subsequently reverted to testes after the exposure. As for treatment of 100 μg/L E2, while the rest of the ovaries maintained morphological stability, percentages of intersexual gonads reached 38%–57%, and none were reverted to testes. Increased mRNA levels of cyp19a, foxl2 and sox9b and decreased mRNA levels of dmrt1, amh, and sox9a were observed during the ovarian development in genetic males. While contrary gene expression profiles were detected during ovary–to–testis transformation. The mRNA levels of all the six genes were increased during the development of intersexual gonads. These results indicated that up–regulation of dmrt1, amh and sox9a is associated with initial ovary–to–intersexual transformation, and suppression of foxl2, cyp19a and sox9b is essential for complete ovary–to–testis recovery in genetic males. This research will help to trace the molecular processes underlying gonadal transformation in teleosts.

Keyword

Takifugu rubripes ovary–to–testis recovery sex–related genes mRNA expression 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Peng Hu
    • 1
    • 2
  • Bin Liu
    • 1
  • Qian Ma
    • 1
    • 2
  • Shufang Liu
    • 1
    • 2
  • Xinfu Liu
    • 1
    Email author
  • Zhimeng Zhuang
    • 1
    • 2
  1. 1.Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoChina
  2. 2.Function Laboratory for Marine Fisheries Science and Food Production ProcessesQingdao National Laboratory for Marine Science and Technology & Function Laboratory for Marine Biology and BiotechnologyQingdaoChina

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