Changes of pigments and lipids composition in Haematococcus pluvialis vegetative cell as affected by monochromatic red light compared with white light
- 25 Downloads
Much attention has been paid on studies of astaxanthin accumulation process in Haematococcus pluvialis industry. However, growth of H. pluvialis in motile vegetative stage is still the most important and problematic part in the whole cultivation process, such as low growth rate and cell yields. Motile vegetative cells are extremely sensitive to various stresses which make it difficult to maintain the cells of this state to grow. Previous reports showed that motile vegetative cells may have higher biomass yields if applied monochromatic red light. However, metabolic responses of these cells are not completely understood, which constraints application of this illumination protocol in industry. The aim of this study was to examine how critical biochemical changes of H. pluvialis motile vegetative cells were affected by red light when compared with white light. Variation of photosynthetic pigments composition and lipids were mainly studied. By comparing growth process of cultures in red light and white light, prominent variation of pigments composition and lipids changes were observed. The results showed that, even though cell proliferation was the same during exponential growth phase, variation of photosynthetic pigment composition and lipids occurred. The final biomass of cell number was higher in red light group than in white light group. The variations were significant different. Increase or decrease of major photosynthetic membrane lipids to some extent did not influence photosynthesis of the vegetative cells during this phase. However, vegetative cells under polychromatic white light other than monochromatic red light need further metabolic process to adjust its pigment composition and lipids, possibly this is energetically and biochemically unfavorable for motile vegetative cells to growth under white light, a light condition normally not considered as a stress.
KeywordHaematococcus pluvialis light quality photosynthetic pigments neutral lipid photosynthetic membrane lipids
Unable to display preview. Download preview PDF.
- Aronsson H, Schöttler M A, Kelly A A, Sundqvist C, Dörmann P, Karim S, Jarvis P. 2008. Monogalactosyldiacylglyceroldeficiency in Arabidopsis affects pigment composition in the prolamellar body and impairs thylakoid membrane energization and photoprotection in leaves. Plant Physiology, 148 (1): 580–592.CrossRefGoogle Scholar
- Fu W Q, Guðmundsson Ó, Paglia G, Herjólfsson G, Andrésson Ó S, Palsson B Ø, Brynjólfsson S. 2013. Enhancement of carotenoid biosynthesis in the green microalga Dunaliella salina with light–emitting diodes and adaptive laboratory evolution. Applied Microbiology and Biotechnology, 97 (6): 2 395–2 403.CrossRefGoogle Scholar
- Gao Z Q, Meng C X, Gao H Z, Zhang X W, Xu D, Su Y F, Wang Y Y, Zhao Y R, Ye N H. 2013. Analysis of mRNA expression profiles of carotenogenesis and astaxanthin production of Haematococcus pluvialis under exogenous 2, 4–epibrassinolide (EBR). Biological Research, 46 (2): 201–206.CrossRefGoogle Scholar
- Gwak Y, Hwang Y S, Wang B B, Kim M, Jeong J, Lee C G, Hu Q, Han D X, Jin E S. 2014. Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis. Journal of Experimental Botany, 65 (15): 4 317–4 334.CrossRefGoogle Scholar
- Kobayashi K, Kondo M, Fukuda H, Nishimura M, Ohta H. 2007. Galactolipid synthesis in chloroplast inner envelope is essential for proper thylakoid biogenesis, photosynthesis, and embryogenesis. Proceedings of the National Academy of Sciences of the United States of America, 104 (43): 17 216–17 221.CrossRefGoogle Scholar
- Li S, Xu J L, Chen J, Chen J J, Zhou C X, Yan X J. 2014. Structural elucidation of co–eluted triglycerides in the marine diatom model organism Thalassiosira pseudonana by ultra–performance liquid chromatography/quadrupole time–of–flight mass spectrometry. Rapid Commun ications in Mass Spectrom etry, 28 (3): 245–255.CrossRefGoogle Scholar
- Mizusawa N, Sakurai I, Kubota H, Wada H. 2008. Role of phosphatidylglycerolin oxygen–evolving complex of photosystem II. In: Allen J F, Gantt E, Golbeck J H, Osmond B eds. Photosynthesis. Energy from the Sun. Springer, Berlin. p.463–466.Google Scholar
- Shah M M R, Liang Y M, Cheng J J, Daroch M. 2016. Astaxanthin–producing green microalga Haematococcus pluvialis: from single cell to high value commercial products. Frontiers in Plant Science, 7: 531.Google Scholar
- Xu J L, Chen D Y, Yan X J, Chen J J, Zhou C X. 2010. Global characterization of the photosynthetic glycerolipids from a marine diatom Stephanodiscus sp. by ultra performance liquid chromatography coupled with electrospray ionization–quadrupole–time of flight mass spectrometry. Analytica Chimica Acta, 663 (1): 60–68.CrossRefGoogle Scholar
- Yamazaki J A, Suzuki T, Maruta E, Kamimura Y. 2005. The stoichiometry and antenna size of the two photosystems in marine green algae, Bryopsis maxima and Ulva pertusa, in relation to the light environment of their natural habitat. Journal of Experimental Botany, 56 (416): 1 517–1 523.CrossRefGoogle Scholar