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Journal of Oceanology and Limnology

, Volume 36, Issue 6, pp 2202–2215 | Cite as

A re-investigation of the bloom-forming unarmored dinoflagellate Karenia longicanalis (syn. Karenia umbella ) from Chinese coastal waters

  • Jianyan Wang (王建艳)
  • Jingyi Cen (岑竞仪)
  • Si Li (李思)
  • Songhui Lü (吕颂辉)
  • Øjvind Moestrup
  • Kin-Ka Chan (陈健嘉)
  • Tao Jiang (江涛)
  • Xiangdong Lei (雷向东)
Article
  • 11 Downloads

Abstract

The dinoflagellate genus Karenia is known for recurrent harmful blooms worldwide. However, species diversity of the genus is generally overlooked owing to the difficulty of identifying small unarmored dinoflagellates. We have established four clonal cultures of Karenia longicanalis isolated from the type locality, Hong Kong harbor (strain HK01) and other three locations along the Chinese coasts (strains YB01, DT01, and NJ01). The morphology of the strain was studied by light and scanning electron microscopy (LM and SEM) and the pigment composition analyzed by high-performance liquid chromatography. We provide the first molecular data of K. longicanalis based on the large subunit (LSU) rRNA gene sequence and internal transcribed spacer (ITS). The four strains showed identical LSU rDNA sequences with a similarity of 99.4% to the holotype of Karenia umbella (strain KUTN05) from Australia. In the ITS phylogeny, the sequence of K. umbella branched between the Chinese strains of K. longicanalis. A careful comparison of the morphology of K. longicanalis and K. umbella reveals the similarity in the diagnostic characters. Differences may appear due to the sample treatment for SEM. We conclude that K. umbella is a junior synonym of K. longicanalis.

Keyword

harmful algal blooms (HABs) phytoplankton morphology phylogeny large subunit (LSU) rRNA internal transcribed spacer (ITS) 

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Notes

Acknowledgement

We sincerely thank Dr. GUAN Wanchun of Wenzhou Medical University for assistance in the field sample collection. Special appreciation is expressed to Dr. LUO Zhaohe of the Third Institute of Oceanography, the State Oceanic Administration for helpful discussions regarding the SEM preparation. We would also like to thank XIA Xiaofei of Beijing Museum of Natural History for his support during SEM photography.

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jianyan Wang (王建艳)
    • 1
  • Jingyi Cen (岑竞仪)
    • 2
    • 3
  • Si Li (李思)
    • 2
    • 3
  • Songhui Lü (吕颂辉)
    • 2
    • 3
  • Øjvind Moestrup
    • 4
  • Kin-Ka Chan (陈健嘉)
    • 5
  • Tao Jiang (江涛)
    • 6
  • Xiangdong Lei (雷向东)
    • 7
  1. 1.Department of Science ResearchBeijing Museum of Natural HistoryBeijingChina
  2. 2.College of Life Science and TechnologyJinan UniversityGuangzhouChina
  3. 3.Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education InstitutesJinan UniversityGuangzhouChina
  4. 4.Marine Biological Section, Department of Biology, University of CopenhagenUniversitetsparken 4Copenhagen ØDenmark
  5. 5.School of Science & TechnologyOpen University of Hong KongKowloon, Hong KongChina
  6. 6.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoChina
  7. 7.Wenzhou Marine Environmental Monitoring Center StationState Oceanic AdministrationWenzhouChina

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