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Journal of Oceanology and Limnology

, Volume 36, Issue 6, pp 2368–2378 | Cite as

Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus

  • Hu Duan (段虎)
  • Songjun Jin (金松君)
  • Fuhua Li (李富花)
  • Xiaojun Zhang (张晓军)
  • Jianhai Xiang (相建海)Email author
Article
  • 18 Downloads

Abstract

As in vertebrates, brains play key roles in rhythmic regulation, neuronal maintenance, differentiation and function, and control of the release of hormones in arthropods. But the structure and functional domains of the brain are still not very clear in crustaceans. In the present study, we reveal the structural details of the brain in the redclaw crayfish using hematoxylin-eosin staining and microscopic examination, firstly. The brain of crayfish is consist of three main parts, namely, protocerebrum, deutocerebrum, and tritocerebrum, including some tracts and commissures, briefly. Secondly, at least 9 kinds of brain cells were identified on the basis of topology and cell shapes, as well as antibody labeling. We also provide morphological details of most cell types, which were previously un-described. In general, four types of glia and three types of neurosecretory cells were described except cluster 9/11 and cluster 10 cells. Glia were categorized into another three main kinds: (1) surface glia; (2) cortex glia; and (3) neuropile glia in addition to astrocytes identified by GFAP labelling. And neurosecretory cells were categorized into I, II and III types based on morphological observation. Finally, cluster 9/11 and 10 cells derived from the brain of crayfish, could be used for primary culture about 7–9 d under the optimized conditions. There results provide a resource for improving the knowledge of the still incompletely defined neuroendocrinology of this species. Using the crayfish as an animal model, we are easy to carry out further research in manipulating their endocrine system, exploring cellular and synaptic mechanisms so much as larval production on a small scale, such as in a cell or tissue.

Keyword

Cherax quadricarinatus brain’s structure brain cells primary culture 

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Notes

Acknowledgement

The authors are grateful to Prof. LIU Haipeng for cell culture training. We would like to give sincere thanks to Dr. GAO Yi and Dr. WANG Quanchao who helped to take some pictures in the manuscript.

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hu Duan (段虎)
    • 1
  • Songjun Jin (金松君)
    • 1
  • Fuhua Li (李富花)
    • 1
    • 2
  • Xiaojun Zhang (张晓军)
    • 1
    • 2
  • Jianhai Xiang (相建海)
    • 1
    • 2
    Email author
  1. 1.Key Laboratory of Experimental Marine Biology, Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdaoChina

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