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Journal of Oceanology and Limnology

, Volume 36, Issue 4, pp 1157–1165 | Cite as

Polyphasic examination on Merismopedia tenuissima CHAB 7021 from Ganjiang River, China revealed the polyphyly of the genus Merismopedia (Cyanobacteria)

  • Liqin Shen (沈丽琴)
  • Suchao Ma (马苏超)
  • Fangfang Cai (蔡芳芳)
  • Gongliang Yu (虞功亮)
  • Shouchun Li (李守淳)
  • Renhui Li (李仁辉)
ICTC-10 Special Issue: Cyanobacteria and cyanotoxins: responses and detection

Abstract

Species in the cyanobacterial genus Merismopedia are present in freshwaters at different trophic levels, with some species even as the components of cyanobacterial blooms. However, species diversity in this genus was not fully verified by molecular investigation and polyphasic taxonomic studies. In this study, Merismopedia-like strain tenuissima CHAB 7021 was isolated from Ganjiang River in Jiangxi Province, China, and polyphasic characterization of this strain was performed by morphological observation, ultrastructural examination, chemical detection of pigments and phylogenetic analysis based on 16S rRNA gene sequences. Morphological identification of the strain was supported by the ultrastructural features, as the tiny species Merismopedia tenuissima Lemmermann. The phylogeny based on 16S rRNA gene sequences revealed at least three clades formed by the strains of Merismopedia. The three M. tenuissima strains including M. tenuissima CHAB 7021 was gathered in clade III with distant relationship to the clade I formed by the six Merismopedia strains including the type species M. punctata, and such a genetic distance may propose Merismopedia tenuissima to separate from Merismopedia genus. However intermixture relationship in between strains of M. punctate and M. glauca in the phylogenetic tree still complicated the taxonomic status in the genus Merismopedia. The process for taxonomic revision in the Merismopedia genus still await for examination and further information on more strains of type species M. punctata.

Keyword

cyanobacteria Merismopedia polyphasic phylogeny taxonomic revision 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Liqin Shen (沈丽琴)
    • 1
  • Suchao Ma (马苏超)
    • 1
  • Fangfang Cai (蔡芳芳)
    • 2
    • 3
  • Gongliang Yu (虞功亮)
    • 2
  • Shouchun Li (李守淳)
    • 1
  • Renhui Li (李仁辉)
    • 2
  1. 1.College of Life ScienceJiangxi Normal UniversityNanchangChina
  2. 2.Key Lab of Algal Biology, Institute of HydrobiologyChinese Academy of SciencesWuhanChina
  3. 3.University of Chinese Academy of SciencesBeijingChina

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