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Journal of Oceanology and Limnology

, Volume 36, Issue 3, pp 913–925 | Cite as

Denitrification potential evaluation of a newly indigenous aerobic denitrifier isolated from largemouth bass Micropterus salmoides culture pond

  • Cuicui Wang (王翠翠)
  • Kai Zhang (张凯)
  • Jun Xie (谢骏)
  • Qigen Liu (刘其根)
  • Deguang Yu (余德光)
  • Guangjun Wang (王广军)
  • Ermeng Yu (郁二蒙)
  • Wangbao Gong (龚望宝)
  • Zhifei Li (李志斐)
Article
  • 25 Downloads

Abstract

This work evaluates the application potential of a new indigenous aerobic denitrifier, strain Pseudomonas CW-2, isolated from a largemouth bass culture pond. The rate of ammonium-N removal by strain CW-2 was approximately 97% at a DO concentration of 5.2 mg/L. Furthermore, when nitrate and ammonia coexisted, the strain gave priority to assimilating ammonia, and thereafter to denitrification. Under optimal cultivation conditions, citrate and acetate were the carbon resources, C/N was 8, dissolved oxygen was 5.2 mg/L, and pH was 7; the removal rate of ammonium reached nearly 90%. The changing patterns of different bacteria in strain CW-2-treated and the control pond water were also compared. Lower levels of ammonia, nitrite, and phosphates were observed in the treated water as compared with the controls. Meanwhile, phylum-level distributions of the bacterial OTUs revealed that Proteobacteria, Bacteroidetes, Planctomycetes, and Nitrospirae continuously changed their relative abundances in relation to carbon and the addition of strain CW-2; this finding implies that the conventional denitrification process was weakened under the effects of carbon or the presence of strain CW-2. We propose that strain CW-2 is a promising organism for the removal of ammonium in intensive fish culture systems, according to our evaluations of its denitrification performance.

Keyword

aerobic denitrification ammonium removal Micropterus salmoides Pseudomonas CW-2 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Cuicui Wang (王翠翠)
    • 1
    • 2
  • Kai Zhang (张凯)
    • 1
  • Jun Xie (谢骏)
    • 1
  • Qigen Liu (刘其根)
    • 2
  • Deguang Yu (余德光)
    • 1
  • Guangjun Wang (王广军)
    • 1
  • Ermeng Yu (郁二蒙)
    • 1
  • Wangbao Gong (龚望宝)
    • 1
  • Zhifei Li (李志斐)
    • 1
  1. 1.Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhouChina
  2. 2.College of Fisheries and Life SciencesShanghai Ocean UniversityShanghaiChina

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