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Journal of Oceanology and Limnology

, Volume 36, Issue 2, pp 317–328 | Cite as

Effects of elevated pCO2 on physiological performance of marine microalgae Dunaliella salina (Chlorophyta, Chlorophyceae

  • Shunxin Hu (胡顺鑫)
  • You Wang (王悠)
  • Ying Wang (王影)
  • Yan Zhao (赵妍)
  • Xinxin Zhang (张鑫鑫)
  • Yongsheng Zhang (张永生)
  • Ming Jiang (姜铭)
  • Xuexi Tang (唐学玺)
Ecology

Abstract

The present study was conducted to determine the effects of elevated pCO2 on growth, photosynthesis, dark respiration and inorganic carbon acquisition in the marine microalga Dunaliella salina. To accomplish this, D. salina was incubated in semi-continuous cultures under present-day CO2 levels (390 μatm, pHNBS: 8.10), predicted year 2100 CO2 levels (1 000 μatm, pHNBS: 7.78) and predicted year 2300 CO2 levels (2 000 μatm, pHNBS: 7.49). Elevated pCO2 significantly enhanced photosynthesis (in terms of gross photosynthetic O2 evolution, effective quantum yield (ΔF/F' m ), photosynthetic efficiency (α), maximum relative electron transport rate (rETRmax) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity) and dark respiration of D. salina, but had insignificant effects on growth. The photosynthetic O2 evolution of D. salina was significantly inhibited by the inhibitors acetazolamide (AZ), ethoxyzolamide (EZ) and 4,4’-diisothiocyanostilbene-2,2′-disulfonate (DIDS), indicating that D. salina is capable of acquiring HCOˉ 3 via extracellular carbonic anhydrase and anion-exchange proteins. Furthermore, the lower inhibition of the photosynthetic O2 evolution at high pCO2 levels by AZ, EZ and DIDS and the decreased carbonic anhydrase showed that carbon concentrating mechanisms were down-regulated at high pCO2. In conclusion, our results show that photosynthesis, dark respiration and CCMs will be affected by the increased pCO2/low pH conditions predicted for the future, but that the responses of D. salina to high pCO2/low pH might be modulated by other environmental factors such as light, nutrients and temperature. Therefore, further studies are needed to determine the interactive effects of pCO2, temperature, light and nutrients on marine microalgae.

Keyword

ocean acidification growth photosynthesis CO2 CCMs Dunaliella salina 

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Notes

Acknowledgment

We thank all of the members of the laboratory and Dr. LIANG in the College of Fisheries of Ocean University of China for their help.

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Shunxin Hu (胡顺鑫)
    • 1
  • You Wang (王悠)
    • 1
  • Ying Wang (王影)
    • 1
  • Yan Zhao (赵妍)
    • 1
  • Xinxin Zhang (张鑫鑫)
    • 1
  • Yongsheng Zhang (张永生)
    • 1
    • 2
  • Ming Jiang (姜铭)
    • 1
  • Xuexi Tang (唐学玺)
    • 1
  1. 1.College of Marine Life ScienceOcean University of ChinaQingdaoChina
  2. 2.Rongcheng Ocean and Fisheries BureauWeihaiChina

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