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Journal of Oceanology and Limnology

, Volume 36, Issue 2, pp 414–426 | Cite as

Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system

  • Chun Wang (王纯)
  • Guoxiang Sun (孙国祥)
  • Shuangshuang Li (李双双)
  • Xian Li (李贤)
  • Ying Liu (刘鹰)Email author
Biology

Abstract

The present study sampled the intestinal content of healthy and unhealthy Atlantic salmon (Salmo salar L.), the ambient water of unhealthy fish, and the biofilter material in the recirculating aquaculture system (RAS) to understand differences in the intestinal microbiota. The V4–V5 regions of the prokaryotic 16S rRNA genes in the samples were analyzed by MiSeq high-throughput sequencing. The fish were adults with no differences in body length or weight. Representative members of the intestinal microbiota were identified. The intestinal microbiota of the healthy fish included Proteobacteria (44.33%), Actinobacteria (17.89%), Bacteroidetes (15.25%), and Firmicutes (9.11%), among which the families Micrococcaceae and Oxalobacteraceae and genera Sphingomonas, Streptomyces, Pedobacter, Janthinobacterium, Burkholderia, and Balneimonas were most abundant. Proteobacteria (70.46%), Bacteroidetes (7.59%), and Firmicutes (7.55%) dominated the microbiota of unhealthy fish, and Chloroflexi (2.71%), and Aliivibrio and Vibrio as well as genera in the family Aeromonadaceae were most strongly represented. Overall, the intestinal hindgut microbiota differed between healthy and unhealthy fish. This study offers a useful tool for monitoring the health status of fish and for screening the utility of probiotics by studying the intestinal microbiota.

Keyword

intestinal microbiota health status Atlantic salmon (Salmo salar L.) recirculating aquaculture system high-throughput pyrosequencing 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Chun Wang (王纯)
    • 1
    • 2
  • Guoxiang Sun (孙国祥)
    • 1
  • Shuangshuang Li (李双双)
    • 3
  • Xian Li (李贤)
    • 1
  • Ying Liu (刘鹰)
    • 1
    Email author
  1. 1.Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.College of Energy and Environmental EngineeringHebei University of EngineeringHandanChina

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