Chinese Journal of Oceanology and Limnology

, Volume 34, Issue 5, pp 1064–1071 | Cite as

Activation of macrophages by an exopolysaccharide isolated from Antarctic Psychrobacter sp. B-3

  • Leiye Yu (玉蕾叶)
  • Guojie Sun (孙国杰)
  • Jingfang Wei (魏景芳)
  • Yingze Wang (王英泽)
  • Chao Du (杜朝)
  • Jiang Li (李江)
Biology
First Online:
Received:
Accepted:

Abstract

An exopolysaccharide (EPS) was isolated and purified from an Antarctic psychrophilic bacterium B-3, identified as Psychrobacter sp., and the activation of RAW264.7 cells by B-3 EPS was investigated. The results show that B-3 EPS, over a certain concentration range, promoted cell viability, nitric oxide production, tumor necrosis factor (TNF)α secretion, and phagocytic ability. Furthermore, TAK-242, an inhibitor of the toll-like receptor 4 (TLR4) significantly reduced nitric oxide production by these cells after stimulation with B-3 EPS. Moreover, B-3 EPS induced p65 phosphorylation and IκBα degradation in these cells. In conclusion, B-3 EPS might have activated RAW264.7 cells by combining with TLR4 on cell surface and triggering activation of NF-κB signaling pathways, implying that this EPS could activate macrophages and regulate initial immune response.

Keyword

exopolysaccharide (EPS) Antarctic bacterium RAW264.7 TLR4 NF-κB 
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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Leiye Yu (玉蕾叶)
    • 1
  • Guojie Sun (孙国杰)
    • 1
  • Jingfang Wei (魏景芳)
    • 1
  • Yingze Wang (王英泽)
    • 1
  • Chao Du (杜朝)
    • 1
  • Jiang Li (李江)
    • 2
  1. 1.College of Bioscience and BioengineeringHebei University of Science and TechnologyHebeiChina
  2. 2.Key Laboratory of Marine Bioactive Substances, First Institute of OceanographyState Oceanic Administration (SOA)QingdaoChina

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