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Glutathione S-transferase (GST) gene expression profiles in two marine bivalves exposed to BDE-47 and their potential molecular mechanisms

  • Fei Li (李斐)
  • Huifeng Wu (吴惠丰)
  • Qing Wang (王清)
  • Xuehua Li (李雪花)
  • Jianmin Zhao (赵建民)
Biology

Abstract

Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobiotics and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs (VpGSTs) and four Mytilus galloprovincialis GSTs (MgGSTs) following exposure to BDE-47. Differential expressions of the seven VpGSTs and four Mg GSTs transcripts were observed, with differences between the hepatopancreas and gills. Among these GSTs, the sigma classes (VpGSTS1, VpGSTS2, VpGSTS3, MgGST1, and MgGST3) were highly expressed in response to BDE-47 exposure, demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of VpGSTs and MgGSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking. Hydrophobic and π were the most often observed interactions between BDE-47 and the GSTs.

Keyword

glutathione S-transferase (GST) Venerupis philippinarum Mytilus galloprovincialis 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) molecular docking biomarker 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Fei Li (李斐)
    • 1
  • Huifeng Wu (吴惠丰)
    • 1
  • Qing Wang (王清)
    • 1
  • Xuehua Li (李雪花)
    • 2
  • Jianmin Zhao (赵建民)
    • 1
  1. 1.Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of SciencesShandong Provincial Key Laboratory of Coastal Zone Environmental ProcessesYantaiChina
  2. 2.Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and TechnologyDalian University of TechnologyDalianChina

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