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Chinese Journal of Oceanology and Limnology

, Volume 31, Issue 1, pp 97–105 | Cite as

Phylogenetic analyses of four species of Ulva and Monostroma grevillei using ITS, rbc L and 18S rDNA sequence data

  • Zhongheng Lin (林中姮)
  • Songdong Shen (沈颂东)Email author
  • Weizhou Chen (陈伟洲)
  • Huihui Li (李慧慧)
Biology

Abstract

Chlorophyta species are common in the southern and northern coastal areas of China. In recent years, frequent green tide incidents in Chinese coastal waters have raised concerns and attracted the attention of scientists. In this paper, we sequenced the 18S rDNA genes, the internal transcribed spacer (ITS) regions and the rbc L genes in seven organisms and obtained 536–566 bp long ITS sequences, 1 377–1 407 bp long rbc L sequences and 1 718–1 761 bp long partial 18S rDNA sequences. The GC base pair content was highest in the ITS regions and lowest in the rbc L genes. The sequencing results showed that the three Ulva prolifera (or U. pertusa) gene sequences from Qingdao and Nan’ao Island were identical. The ITS, 18S rDNA and rbc L genes in U. prolifera and U. pertusa from different sea areas in China were unchanged by geographic distance. U. flexuosa had the least evolutionary distance from U. californica in both the ITS regions (0.009) and the 18S rDNA (0.002). These data verified that Ulva and Enteromorpha are not separate genera.

Keyword

Ulva ITS region rbc18S rDNA phylogeny sequences analysis 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Zhongheng Lin (林中姮)
    • 1
  • Songdong Shen (沈颂东)
    • 1
    Email author
  • Weizhou Chen (陈伟洲)
    • 2
  • Huihui Li (李慧慧)
    • 1
  1. 1.Department of Cell Biology, School of Biology and Basic Medical SciencesSoochow UniversitySuzhouChina
  2. 2.Institute of Marine BiologyShantou UniversityShantouChina

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