Chinese Journal of Oceanology and Limnology

, Volume 30, Issue 5, pp 724–730 | Cite as

Extract of Meretrix meretrix Linnaeus induces angiogenesis in vitro and activates endothelial nitric oxide synthase

  • Ming Liu (刘明)
  • Jianteng Wei (魏鉴腾)
  • Hui Wang (王惠)
  • Lili Ding (丁丽丽)
  • Yuyan Zhang (张玉艳)
  • Xiukun Lin (林秀坤)
Biology
First Online:
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Accepted:
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Abstract

Meretrix meretrix Linnaeus has long been used as traditional Chinese medicine in oriental medicine. The angiogentic activity of the extract of M. meretrix was investigated in this study, using human umbilical vein endothelial cells (HUVECs). Extract of M. meretrix Linnaeus (AFG-25) was prepared with acetone and ethanol precipitation, and further separated by Sephadex G-25 column. The results show that AFG-25 promoted proliferation, migration, and capillary-like tube formation in HUVECs, and in the presence of eNOS inhibitor NMA, the tube formation induced by AFG-25 is inhibited significantly. Moreover, AFG-25 could also promote the activation of endothelial nitric oxide synthase (eNOS) and the resultant elevation of nitric oxide (NO) production. The results suggested that M. meretrix contains active ingredients with angiogentic activity and eNOS/NO signal pathway is in part involved in the proangiogenesis effect induced by AFG-25.

Keyword

Meretrix meretrix Linnaeus angiogenesis NOS nitric oxide (NO) 
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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Ming Liu (刘明)
    • 1
  • Jianteng Wei (魏鉴腾)
    • 1
    • 2
  • Hui Wang (王惠)
    • 1
    • 2
  • Lili Ding (丁丽丽)
    • 1
    • 2
  • Yuyan Zhang (张玉艳)
    • 1
  • Xiukun Lin (林秀坤)
    • 1
    • 3
  1. 1.Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingChina
  3. 3.Department of PharmacologyCapital Medical UniversityBeijingChina

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