Chinese Journal of Oceanology and Limnology

, Volume 29, Issue 5, pp 952–957 | Cite as

Gene cloning and prokaryotic expression of recombinant outer membrane protein from Vibrio parahaemolyticus

  • Ye Yuan (袁野)
  • Xiuli Wang (王秀利)
  • Sheping Guo (郭设平)
  • Xuemei Qiu (仇雪梅)
Biology
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Abstract

Gram-negative Vibrio parahaemolyticus is a common pathogen in humans and marine animals. The outer membrane protein of bacteria plays an important role in the infection and pathogenicity to the host. Thus, the outer membrane proteins are an ideal target for vaccines. We amplified a complete outer membrane protein gene (ompW) from V. parahaemolyticus ATCC 17802. We then cloned and expressed the gene into Escherichia coli BL21 (DE3) cells. The gene coded for a protein that was 42.78 kDa. We purified the protein using Ni-NTA affinity chromatography and Anti-His antibody Western blotting, respectively. Our results provide a basis for future application of the OmpW protein as a vaccine candidate against infection by V. parahaemolyticus. In addition, the purified OmpW protein can be used for further functional and structural studies.

Keyword

Vibrio parahaemolyticus outer membrane protein (OmpW) cloning prokaryotic expression protein characterization recombinant proteins 
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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Ye Yuan (袁野)
    • 1
  • Xiuli Wang (王秀利)
    • 1
  • Sheping Guo (郭设平)
    • 1
  • Xuemei Qiu (仇雪梅)
    • 1
  1. 1.College of Life Sciences and BiotechnologyDalian Ocean UniversityDalianChina

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