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Chinese Journal of Oceanology and Limnology

, Volume 28, Issue 4, pp 808–814 | Cite as

PCR-DGGE analysis of intestinal bacteria and effect of Bacillus spp. on intestinal microbial diversity in kuruma shrimp (Marsupenaeus japonicus)

  • Huaide Liu (刘淮德)
  • Mei Liu (刘梅)
  • Baojie Wang (王宝杰)
  • Keyong Jiang (蒋克勇)
  • Shan) Jiang (姜珊)
  • Shujuan Sun (孙姝娟)
  • Lei Wang (王雷)
Article

Abstract

In this study, the intestinal microbiota of kuruma shrimp (Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacillus spp. on intestinal microbial diversity. Samples of the intestines of kuruma shrimp fed normal feed and Bacillus spp. amended feed. PCR and denaturing gradient gel electrophoresis (DGGE) analyses were then performed on DNA extracted directly from the guts. Population fingerprints of the predominant organisms were generated by DGGE analysis of the universal V3 16S rDNA amplicons, and distinct bands in the gels were sequenced. The results suggested that the gut of kuruma shrimp was dominated by Vibrio sp. and uncultured gamma proteobacterium. Overall, the results of this study suggest that PCR-DGGE is a possible method of studying the intestinal microbial diversity of shrimp.

Keyword

intestinal microbial diversity PCR-DGGE Kuruma shrimp 

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Copyright information

© Chinese Society for Oceanology and Limnology, Science Press and Springer Berlin Heidelberg 2010

Authors and Affiliations

  • Huaide Liu (刘淮德)
    • 1
    • 2
  • Mei Liu (刘梅)
    • 1
  • Baojie Wang (王宝杰)
    • 1
    • 2
  • Keyong Jiang (蒋克勇)
    • 1
  • Shan) Jiang (姜珊)
    • 1
    • 2
  • Shujuan Sun (孙姝娟)
    • 1
    • 2
  • Lei Wang (王雷)
    • 1
  1. 1.Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingChina

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