In situ optical measurements of bacterial endospore breakdown in a shock tube
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The interaction of endospore-laden bioaerosols and shock waves is monitored with a combination of laser absorption and scattering. Tests are performed in the Stanford aerosol shock tube for post-shock temperatures ranging from 400–1100 K. In situ laser measurements at 266 and 665 nm provide a real-time monitor of endospore morphology. Scatter of visible light measures the integrity of endospore structure, while absorption of UV light provides a monitor of biochemicals released by endospore rupture. For post-shock temperatures greater than 750 K endospore morphological breakdown is observed. A simple theoretical model is employed to quantify the optical measurements, and mechanisms leading to the observed data are discussed.
KeywordsShock Wave Shock Tube Incident Shock Wave Breakdown Rate Diaphragm Rupture
This work was supported by the Defense Threat Reduction Agency via grant AB07TAS014, administered by the Army Research Office under grant 51532CHCBB with Dr. Jennifer Becker of the Chemical Sciences Division as contract monitor.