Optimized coherence parameters for high-resolution holographic microscopy
- First Online:
A holographic in-line microscope setup with a glass sample carrier commonly uses a coherent laser light source, with the disadvantage of an incomplete suppression of disturbing interferences and coherence-induced noise.
We use temporally partially coherent and spatially partially coherent illumination in the micrometer range of 5 to 100 μm generated by spectrally and spatially filtered light-emitting diodes (LEDs) and tungsten filament lamps. It is shown how errors in reconstructed holograms are decreased by a factor of more than two and the spatial resolution reaches the limit as determined by the numerical aperture. A straightforward model is developed to investigate the effects of partial coherence. The optimization of the coherence parameters improves the imaging quality up to the limit of a conventional microscope.
Unable to display preview. Download preview PDF.
- 1.J.W. Goodman, Speckle Phenomena in Optics (Roberts & Company, Englewood, 2007) Google Scholar
- 6.G.I. Kosourov, I.N. Kalinkina, M.P. Golovei, Pisma ZhETF 4, 84 (1966) Google Scholar
- 16.E. Hecht, Optik (Addison-Wesley, Bonn, 1992) Google Scholar
- 17.J. Popp, V.V. Tuchin, A. Chiou, S. Heinemann (eds.), Handbook of Biophotonics, Basics and Techniques, vol. 1 (Wiley-VCH, Weinheim, 2011), p. 268 Google Scholar
- 20.J.W. Goodman, Introduction to Fourier Optics, 2nd edn. (McGraw-Hill, New York, 1996) Google Scholar