Applied Physics B

, Volume 87, Issue 4, pp 629–634

Tunable mid-infrared, high-energy femtosecond laser source for glyco-protein structure analysis

  • T. Imahoko
  • K. Takasago
  • T. Sumiyoshi
  • H. Sekita
  • K. Takahashi
  • M. Obara
Article

Abstract

We have developed a 6–12 μm mid-infrared (MIR) femtosecond laser source for glyco-protein structure analysis. The MIR femtosecond laser pulses are generated by a differential frequency generation (DFG) configuration with a combination of Ti:sapphire based regeneratively amplified femtosecond laser pulses (780 nm, 160 fs, 1 mJ) and a β-BaB2O4 (BBO) based optical parametric amplifier (OPA). The MIR pulse energy exceeds 4.5 μJ, where a glyco-protein molecule has resonant absorption lines due to the vibrational–rotational transitions. The pulse width is estimated to be less than 1 ps according to the cross correlation measurement between the two OPA output pulses. Using the MIR femtosecond laser pulses, we demonstrated photo-dissociation of the sialyl Lewis X (sLeX) proton added ion, which is the first time to the best of our knowledge.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • T. Imahoko
    • 1
    • 3
  • K. Takasago
    • 1
  • T. Sumiyoshi
    • 1
  • H. Sekita
    • 1
  • K. Takahashi
    • 2
  • M. Obara
    • 3
  1. 1.Cyber Laser Inc.TokyoJapan
  2. 2.Computational Biology Research CenterAIST Tokyo WaterfrontTokyoJapan
  3. 3.School of Integrated Design EngineeringKeio UniversityYokohama-shiJapan

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