Simultaneous time- and spectrum-resolved multifocal multiphoton microscopy
- 112 Downloads
We present a novel multifocal multiphoton microscope that is based on a sampling imaging technique using a microlens array, a prism for two-dimensionalspectral dispersion, and a specially designed streak camera to provide simultaneous time- and spectrum-resolved fluorescence microscopy. We split the near-infrared light of a mode-locked titanium:sapphire femtosecond laser into an array of beams that are transformed into an array of high-aperture foci at the sample. A time- and spectrum-resolved image of 3×3 foci on the sample can be obtained with a snapshot. By translating the sample stage laterally and axially and implementing a dedicated image reconstruction algorithm in the control system of the instrument, we demonstrate the acquisition of a five-dimensional data set combining lifetime and spectral resolutions in biological fluorescence imaging.
Unable to display preview. Download preview PDF.
- 3.A. Gillenwater, R. Jacob, R. Ganeshappa, B. Kemp, A.K. El-Naggar, J.L. Palmer, G. Clayman, M.F. Mitchell, R. Richards-Kortum, Arch. Otolaryngol. 124, 1251 (1998)Google Scholar
- 16.L. Liu, J. Qu, Z. Lin, D. Chen, T. Hu, B. Guo, H. Niu, Acta Opt. Sin. 26, 373 (2006)Google Scholar
- 17.L. Liu, J. Qu, Z. Lin, D. Chen, G. Xu, T. Hu, B. Guo, H. Niu, Acta Phys. Sin. (2006), unpublishedGoogle Scholar