Applied Physics B

, Volume 77, Issue 4, pp 391–397

Femtosecond laser-induced-breakdown spectrometry for Ca2+ analysis of biological samples with high spatial resolution

  • A. Assion
  • M. Wollenhaupt
  • L. Haag
  • F. Mayorov
  • C. Sarpe-Tudoran
  • M. Winter
  • U. Kutschera
  • T. Baumert
Regular Paper

Abstract

We describe an analytical method for element-specific in situ investigations of biological samples with high spatial resolution, using laser-induced-breakdown spectroscopy (LIBS). The prerequisites for spatially highly resolved LIBS are an appropriate analytical performance in combination with precise micro-ablation conditions, both laterally and axially. In order to identify the best suitable laser source, the analytical performance and the ablation process were studied for ns-LIBS and fs-LIBS. The analytical performance was studied on an aqueous solution of CaCl2, where water served as a first substitute for biological material. The ablation process was investigated in the outer epidermal wall of a sunflower seedling stem. Besides direct measurements, the physiology of the sunflower seedling can be used in order to estimate the ablation depth. Our results show that analytical measurements with high spatial resolution can be performed on biological samples using fs-LIBS. This was demonstrated by in situ measurements of wall-associated calcium ion (Ca2+) distributions within the peripheral cell wall of the sunflower seedling (Helianthus annuus L.) stem. In this biological sample an axial resolution of about 100 nm was achieved.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    D.A. Rusak, B.C. Castle, B.W. Smith, J.D. Winefordner: Trends Anal. Chem. 17, 453 (1998) CrossRefGoogle Scholar
  2. 2.
    D. Kossakovski, J.L. Beauchamp: Anal. Chem. 72, 4731 (2000) CrossRefGoogle Scholar
  3. 3.
    L. St-Onge, M. Sabsabi: Spectrochim. Acta Part B: At. Spectrosc. 55, 299 (2000) ADSCrossRefGoogle Scholar
  4. 4.
    D. Romero, J.J. Laserna: Spectrochim. Acta Part B 55, 1241 (2000) ADSCrossRefGoogle Scholar
  5. 5.
    J.M. Vadillo, C.C. Garcia, S. Palanco, J.J. Laserna: J. Anal. At. Spectrom. 13, 793 (1998) CrossRefGoogle Scholar
  6. 6.
    A. Kaiser, B. Rethdeld, M. Vicanek, G. Simon: Phys. Rev. B 61, 11437 (2000) ADSCrossRefGoogle Scholar
  7. 7.
    B.C. Stuart, M.D. Feit, A.M. Rubenchik, B.W. Shore, M.D. Perry: Phys. Rev. Lett. 74, 2248 (1995) ADSCrossRefGoogle Scholar
  8. 8.
    B.C. Stuart, M.D. Feit, S. Herman, A.M. Rubenchik, B.W. Shore, M.D. Perry: Phys. Rev. B 53, 1749 (1996) ADSCrossRefGoogle Scholar
  9. 9.
    J. Krüger, W. Kautek, M. Lenzner, S. Sartania, C. Spielmann, F. Krausz: Appl. Surf. Sci. 127129, 892 (1998) Google Scholar
  10. 10.
    R. Stoin, D. Ashkenasi, A. Rosenfeld, E. Campbell: Phys. Rev. B 62, 13167 (2000) ADSCrossRefGoogle Scholar
  11. 11.
    A.C. Tien, S. Backus, H.C. Kapteyn, M.M. Murnane, G. Mourou: Phys. Rev. Lett. 82, 3883 (1999) ADSCrossRefGoogle Scholar
  12. 12.
    J. Bonse, M. Geuss, S. Baudach, H. Sturm, W. Kautek: Appl. Phys. A 69, 399 (1999) ADSCrossRefGoogle Scholar
  13. 13.
    X. Zhu, D.M. Villeneuve, A.Y. Naumov, S. Nikumb, P.B. Corkum: Appl. Surf. Sci. 152, 138 (1999) ADSCrossRefGoogle Scholar
  14. 14.
    O. Lehmann, M. Stuke: Appl. Phys. Lett. 67, 338 (1995) CrossRefGoogle Scholar
  15. 15.
    C. Momma, B.N. Chichkov, S. Nolte, F. von Alvensleben, A. Tünnermann, H. Welling, B. Welleghausen: Opt. Commun. 129, 134 (1996) ADSCrossRefGoogle Scholar
  16. 16.
    D. von der Linde, K. Sokolowski-Tinten, J. Bialkowski: Appl. Surf. Sci. 109110, 1 (1997) Google Scholar
  17. 17.
    B.N. Chichkov, C. Momma, F. von Alvensleben, A. Tünnermann: Appl. Phys. A 63, 109 (1996) ADSCrossRefGoogle Scholar
  18. 18.
    M.D. Feit, A.M. Rubenchik, B.-M. Kim, L.B. da Silva, M.D. Perry: Appl. Surf. Sci. 127129, 869 (1998) Google Scholar
  19. 19.
    B.-M. Kim, M.D. Feit, A.M. Rubenchik, B.M. Mammini, L.B. da Silva: Appl. Surf. Sci. 127129, 857 (1998) Google Scholar
  20. 20.
    F.H. Loesel, M.H. Niemz, J.F. Bille, T. Juhasz: J. Quantum Electron. 32, 1717 (1996) ADSCrossRefGoogle Scholar
  21. 21.
    A.A. Oraevsky, L.B. da Silva, A.M. Rubenchik, M.D. Feit, M.E. Glinsky, M.D. Perry, B.M. Mammini, W. Small, B.C. Stuart: IEEE J. Sel. Top. Quantum Electron. 2, 801 (1996) CrossRefGoogle Scholar
  22. 22.
    V. Margetic, A. Pakulev, A. Stockhaus, M. Bolshov, K. Niemax, R. Hergenröder: Spectrochim. Acta Part B 55, 1771 (2000) ADSCrossRefGoogle Scholar
  23. 23.
    B. Le Drogoff, J. Margot, M. Chaker, M. Sabsabi, O. Barthélemy, T.W. Johnston, S. Laville, F. Vidal, Y. von Kaenel: Spectrochim. Acta Part B 56, 987 (2001) ADSCrossRefGoogle Scholar
  24. 24.
    K.L. Eland, D.N. Stratis, D.M. Gold, S.R. Goode, S.M. Angel: Appl. Spectrosc. 55, 286 (2001) ADSCrossRefGoogle Scholar
  25. 25.
    R. Netz, T. Feurer, R. Wolleschensky, R.A. Sauerbrey: Appl. Phys. B 70, 833 (2000) ADSCrossRefGoogle Scholar
  26. 26.
    U. Kutschera: Planta 181, 316 (1990) CrossRefGoogle Scholar
  27. 27.
    J. Deitche, M. Kempe, W. Rudolph: J. Microsc. 174, 69 (1994) CrossRefGoogle Scholar
  28. 28.
    E.J.B. Pawley: Handbook of Biological Confocal Microscopy (Plenum, New York 1995) Google Scholar
  29. 29.
    T.R. Corle, G.S. Kino: Confocal Scanning Optical Microscopy and Related Imaging Systems (Academic, San Diego, CA 1996) Google Scholar
  30. 30.
    B. Le Drogoff, J. Margot, M. Chaker, M. Sabsabi, O. Barthélemy, T.W. Johnston, S. Laville, F. Vidal, Y. von Kaenel: Spectrochim. Acta Part B 56, 987 (2001) ADSCrossRefGoogle Scholar
  31. 31.
    C.H. Fan, J. Sun, J.P. Longtin: J. Appl. Phys. 91, 2530 (2002) ADSCrossRefGoogle Scholar
  32. 32.
    J. Noack, A. Vogel: IEEE J. Quantum Electron. 35, 1156 (1999) ADSCrossRefGoogle Scholar
  33. 33.
    A. Vogel, J. Noack, K. Nahen, D. Theisen, S. Busch, U. Parlitz, D.X. Hammer, G.D. Noojin, B.A. Rockwell, R. Birngruber: Appl. Phys. B 68, 271 (1999) ADSCrossRefGoogle Scholar
  34. 34.
    U. Kutschera: Rev. Bras. Fisiol. Veg. 65, 66 (2000)Google Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • A. Assion
    • 1
  • M. Wollenhaupt
    • 1
  • L. Haag
    • 1
  • F. Mayorov
    • 1
  • C. Sarpe-Tudoran
    • 1
  • M. Winter
    • 1
  • U. Kutschera
    • 2
  • T. Baumert
    • 1
  1. 1.Fachbereich für Naturwissenschaften und Center of Interdisciplinary Nanostructure Science and Technology (CINSaT), Institut für PhysikUniversität KasselKasselGermany
  2. 2.Fachbereich für Naturwissenschaften und Center of Interdisciplinary Nanostructure Science and Technology (CINSaT), Institut für BiologieUniversität KasselKasselGermany

Personalised recommendations