Fast and highly selective separation of His-tagged proteins by Ni2+-carrying magnetic core–shell nanoparticles
- 20 Downloads
In this study, core–shell Fe3O4@Au nanoparticles with good chemical stability and tunable particle size were synthesized via Au–S bonding, which overcomes the challenges encountered for the reported preparation of Fe3O4@Au. The surface of nanoparticles was investigated by TEM, XRD, XPS, FT-IR, VSM and DLS which characterized the size, morphology, chemical lattice, elemental analysis, functional groups, magnetic strength, and size distribution. The results showed that Fe3O4@Au/NTA-Ni2+ magnetic nanocomposites were spherical with an average diameter of 469.5 nm, and superparamagnetic with saturation magnetization of 7 emu/g. The magnetic nanocomposites were directly utilized for one-step purification of His-tagged proteins from Description Escherichia coli lysate. The as-separated proteins were qualitatively validated by gel analysis and quantitatively measured by UV–visible spectroscopy. The binding capacity of Fe3O4@Au/NTA-Ni2+ towards His-tagged proteins was 48.3 mg/g. The target proteins were separated with high purity and separation efficiency up to 96.6%. Their specificity and enrichment ability were maintained during six cycles of adsorption–elution operations. The advantages of this novel nanoparticle can open a window for the multifunction of nanomaterials and their biological application.
This study was funded by National Natural Science Foundation of China (21401051), Hubei Province Natural Science Fund Project (2014CFB595), and Hubei Province Outstanding youth science and technology innovation team in institutions of higher education (T201705).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.E. Dekking, V.H.J.V.D. Velden, S. Böttcher, M. Brüggemann, E. Sonneveld, A. Koning-Goedheer, N. Boeckx, P. Lucio, L. Sedek, T. Szczepański, T. Kalina, M. Kovac, P. Evans, P.G. Hoogeveen, J. Flores-Montero, A. Orfao, W.M. Comans-Bitter, F.J.T. Staal, J.J.M.V. Dongen, Best Pract. Res. Clin. Haematol. 23, 333–345 (2010)Google Scholar
- 6.J.R. Kintzing, M.V. Filsinger Interrante, J.R. Cochran, Trends Pharmacol. Sci. 37, 993 (2016)Google Scholar
- 11.Y.H. Wu, G.X. Chang, Y.B. Zhao, Y. Zhang, J. Nanopart. Res. 16, 1–7 (2014)Google Scholar
- 15.M. Zhang, X.W. He, L.X. Chen, Y.K. Zhang, Nanotechnology 22, 1–8 (2011)Google Scholar
- 41.Y. Zhou, D.D. Yan, S.F. Yuan, Y.W. Chen, Emmanuella E. Fletcher, H.F. Shi, B.X. Han, Protein Express. Purif. 144, 5–11 (2018)Google Scholar