Applied Physics A

, 124:222 | Cite as

One-step production of phage–silicon nanoparticles by PLAL as fluorescent nanoprobes for cell identification

  • Laura M. De Plano
  • Santi Scibilia
  • Maria Giovanna Rizzo
  • Sara Crea
  • Domenico Franco
  • Angela M. Mezzasalma
  • Salvatore P. P. Guglielmino
Article
  • 53 Downloads

Abstract

Silicon nanoparticles (SiNPs) are widely used as promising nanoplatform owing to their high specific surface area, optical properties and biocompatibility. Silicon nanoparticles find possible application in biomedical environment for their potential quantum effects and the functionalization with biomaterials, too. In this work, we propose a new approach for bio-functionalization of SiNPs and M13-engineered bacteriophage, displaying specific peptides that selectively recognize peripheral blood mononuclear cells (PBMC). The “one-step” functionalization is conducted during the laser ablation of silicon plate in buffer solution with engineered bacteriophages, to obtain SiNPs binding bacteriophages (phage–SiNPs). The interaction between SiNPs and bacteriophage is investigated. Particularly, the optical and morphological characterizations of phage–SiNPs are performed by UV–Vis spectroscopy, scanning electron microscopy operating in transmission mode (STEM) and X-ray spectroscopy (EDX). The functionality of phage–SiNPs is investigated through the photoemissive properties in recognition test on PBMC. Our results showed that phage–SiNPs maintain the capability and the activity to bind PBMC within 30 min. The fluorescence of phage–SiNPs allowed to obtain an optical signal on cell type targets. Finally, the proposed strategy demonstrated its potential use in in vitro applications and could be exploited to realize an optical biosensor to detect a specific target.

Notes

Acknowledgements

The authors thank Dr. F. Barreca and Prof. F. Neri for helping with scanning electron microscopy operating in transmission mode (STEM) measurements.

References

  1. 1.
    M.A. Walling, J.A. Novak, J.R.E. Shepard, Int. J. Mol. Sci. 10, 441–491 (2009)CrossRefGoogle Scholar
  2. 2.
    W.C.W. Chan, D.J. Maxwell, X.H. Gao, R.E. Bailey, M.Y. Han, S.M. Nie, Curr. Opin. Biotechnol. 13, 40–46 (2002)CrossRefGoogle Scholar
  3. 3.
    A.M. Derfus, W.C.W. Chan, S.N. Bhatia, Nano Lett. 4, 11–18 (2004)ADSCrossRefGoogle Scholar
  4. 4.
    P. Chewchinda, O. Odawara, H. wada, CheM 3, 81–86 (2016)Google Scholar
  5. 5.
    R. Intartaglia, K. Bagga, A. Genovese, A. Athanassiou, R. Cingolani, A. Diaspro, F. Brandi, Chem. Chem. Phys. 14, 15406 (2012)CrossRefGoogle Scholar
  6. 6.
    K. Wang, X. He, X. Yang, H. Shi, Acc. Chem. Res. 46(7), 1367–1376 (2013)CrossRefGoogle Scholar
  7. 7.
    I.L. Medintz, H.T. Uyeda, E.R. Goldman, H. Mattoussi, Nat. Mater. 4, 435–446 (2005)ADSCrossRefGoogle Scholar
  8. 8.
    N.S.K. Gunda, M. Singh, L. Norman, K. Kaur, S.K. Mitra, Appl. Surf. Sci. 305, 522–530 (2014)ADSCrossRefGoogle Scholar
  9. 9.
    V. Cauda, C. Argyo, T. Bein, J. Mater. Chem. 20, 8693–8699 (2010)CrossRefGoogle Scholar
  10. 10.
    S.V. Rao, G.K. Podagatlapalli, S. Hamad, J. Nanosci. Nanotechnol. 14(2), 1364–1388 (2014)CrossRefGoogle Scholar
  11. 11.
    D. Joshi, R.K. Soni, Appl. Phys. A 116, 635–664 (2014)ADSCrossRefGoogle Scholar
  12. 12.
    K. Bagga, A. Barchanski, R. Intartaglia, S. Dante, R. Marotta, A. Diaspro, C.L. Sajti, F. Brandi, Laser Phys. Lett. 10, 065603 (2013)ADSCrossRefGoogle Scholar
  13. 13.
    T.C. Lee, K. Yusoffa, S. Nathan, W.S. Tan, J. Virol. Methods. 136(1–2), 224–229 (2006)CrossRefGoogle Scholar
  14. 14.
    S. Petersen, S. Barcikowski, Adv. Funct. Mater. 19, 1167–1172 (2009)CrossRefGoogle Scholar
  15. 15.
    V.A. Petrenko, G.P. Smith, Protein Eng. 13(8), 589–592 (2000)CrossRefGoogle Scholar
  16. 16.
    C.F. Barbas III, D.R. Burton, J.K. Scott, G.J. Silverman, Phage Display, A Laboratory Manual. (Cold Spring Harbor Lab. Press, Woodbury, 2001)Google Scholar
  17. 17.
    S. Scibilia, G. Lentini, E. Fazio, D. Franco, F. Neri, A.M. Mezzasalma, S.P.P. Guglielmino, Sens. Bio-Sens. Res. 7, 146–152 (2016)CrossRefGoogle Scholar
  18. 18.
    L.M. De Plano, S. Carnazza, G.M.L. Messina, M.G. Rizzo, G. Marletta, S.P.P. Guglielmino, Colloids Surf. B 157, 473–480 (2017)CrossRefGoogle Scholar
  19. 19.
    G. Lentini, E. Fazio, F. Calabrese, L.M. DePlano, M. Puliafico, D. Franco, M.S. Nicolò, S. Carnazza, S.Trusso,A. Allegra, F. Neri, C. Musolino, S.P.P. Guglielmino, Biosens. Bioelectron. 74, 398–405 (2015)CrossRefGoogle Scholar
  20. 20.
    E. Fazio, A. Cacciola, A.M. Mezzasalma, G. Mondio, F. Neri, R. Saija, J. Quant. Spectro. Rad. Trans. 124, 86–93 (2013)ADSCrossRefGoogle Scholar
  21. 21.
    D.L. Jaye, C.M. Geigerman, R.E. Fuller, A. Akyildiz, A. Parkos, J. Immunol. Methods 295, 119–127 (2004)CrossRefGoogle Scholar
  22. 22.
    J.C. Butler, T. Angelini, J.X. Tang, G.C.L. Wong, Phys. Rev. Lett. 91, 028301 (2003)ADSCrossRefGoogle Scholar
  23. 23.
    K.N. Parent, S.M. Doyle, E. Anderson, C.M. Teschke, Virology 340, 33–45 (2005)CrossRefGoogle Scholar
  24. 24.
    T.G. Ulusoy Ghobadi, A. Ghobadi, T. Okyay, K. Topalli, A.K. Okyay, RSC Adv. 6, 112520 (2016)CrossRefGoogle Scholar
  25. 25.
    J.R. Brigati, V.A. Petrenko, Anal. Bioanal. Chem. 382, 1346–1350 (2005)CrossRefGoogle Scholar
  26. 26.
    M. Coen, R. Lehmann, P. Groning, M. Bielmann, C. Galli, L. Schlapbach, J. Colloid Interface Sci. 233, 180–189 (2001)ADSCrossRefGoogle Scholar
  27. 27.
    F.X. Schmid, In Encyclopedia Life Sciences, Introductory Articles, ed. by R. Bridgewater (Wiley, 2001), pp. 1–4.  https://doi.org/10.1038/npg.els.0003142
  28. 28.
    S.A. Overman, P. Bondr, N.C. Maiti, G.J. Thomas Jr, Biochemistry 44, 3091–3100 (2005)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Laura M. De Plano
    • 1
  • Santi Scibilia
    • 2
  • Maria Giovanna Rizzo
    • 1
  • Sara Crea
    • 1
  • Domenico Franco
    • 1
  • Angela M. Mezzasalma
    • 2
  • Salvatore P. P. Guglielmino
    • 1
  1. 1.Department of Chemical, Biological, Pharmaceutical and Environmental SciencesUniversity of MessinaMessinaItaly
  2. 2.Department of Mathematics, Informatics, Physics and Earth SciencesUniversity of MessinaMessinaItaly

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