Influence of ceramide on the internal structure and hydration of the phospholipid bilayer studied by neutron and X-ray scattering
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Small angle neutron scattering (SANS), neutron diffraction and X-ray powder diffraction were used to investigate influence of N-stearoyl phytosphingosine (CER[NP]) and α-hydroxy-N-stearoyl phytosphingosine (CER[AP]) on the internal structure and hydration of DMPC membrane in fully and partly hydrated states at T = 30 °C. Application of Fourier analysis for diffraction data and model calculations for the SANS data evidence that addition of both CER[NP] and CER[AP] in small concentrations promotes significant changes in the organization of DMPC bilayers, such as the increase of the hydrophobic core region. SANS data evidence a decrease in the average radius and polydispersity of the vesicles that can be ascribed to hydrogen bonds interactions that favor tight lipid packing with a compact, more rigid character.
The authors wish to thank Cosmoferm B.V., Lipoid (Ludwigshafen, Germany) and Degussa, Goldschmidt AG (Essen, Germany) for the gift of chemicals. Thanks are also extended to DESY, HMI, and BNSC facility for financial support during scattering experiments runs. This study was supported by RFBR grants 12-02-00686, 12-02-12045, and 13-01-00060.