Applied Physics A

, Volume 101, Issue 2, pp 423–428 | Cite as

Micro-channel fabrication by femtosecond laser to arrange neuronal cells on multi-electrode arrays

  • Chie Hosokawa
  • Suguru N. Kudoh
  • Mariko Suzuki
  • Ai Kiyohara
  • Yoichiroh Hosokawa
  • Kazunori Okano
  • Hiroshi Masuhara
  • Takahisa Taguchi
Article

Abstract

We propose and demonstrate micro-channel fabrication by femtosecond-laser processing to arrange neuronal cells on multi-electrode arrays (MEAs). When neuronal cells were seeded in the micro-channels on MEAs, they elongated their neurites along the channel and finally formed the network patterned on MEAs. The spontaneous electrical activity of the neuronal networks on MEAs was evaluated by using electrophysiological systems, indicating that patterned network was functionally activated along the channels. The present method is applicable to analyze network dynamics of living neuronal cells patterned artificially.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Chie Hosokawa
    • 1
    • 2
    • 4
  • Suguru N. Kudoh
    • 1
    • 5
  • Mariko Suzuki
    • 1
    • 4
  • Ai Kiyohara
    • 1
    • 5
  • Yoichiroh Hosokawa
    • 3
  • Kazunori Okano
    • 3
  • Hiroshi Masuhara
    • 3
    • 6
  • Takahisa Taguchi
    • 1
    • 4
  1. 1.Research Institute for Cell EngineeringNational Institute of Advanced Industrial Science and Technology (AIST)OsakaJapan
  2. 2.PRESTOJapan Science and Technology Agency (JST)SaitamaJapan
  3. 3.Graduate School of Materials ScienceNara Institute of Science and Technology (NAIST)NaraJapan
  4. 4.Health Research InstituteNational Institute of Advanced Industrial Science and Technology (AIST)IkedaJapan
  5. 5.Department of Human System Interaction, School of Science and TechnologyKwansei Gakuin UniversityHyogoJapan
  6. 6.Department of Applied Chemistry and Institute of Molecular ScienceNational Chiao Tung UniversityHsinchuTaiwan

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