Applied Physics A

, Volume 93, Issue 1, pp 57–63 | Cite as

Femtosecond laser modification of living neuronal network

  • Chie Hosokawa
  • Suguru N. Kudoh
  • Ai Kiyohara
  • Yoichiroh Hosokawa
  • Kazunori Okano
  • Hiroshi Masuhara
  • Takahisa Taguchi
Article

Abstract

An all-wet femtosecond laser microprocessing technique was utilized for patterning and cutting functional network of living neuronal cells on a multi-electrode dish (MED). The neuronal cells cultured on a source substrate were transferred onto an electrode in a MED probe in solution by utilizing a femtosecond laser-induced impulsive force and a pattern of neuronal cells were formed on the MED probe. The cellular activity of the detached neurons was supported that neurites could be regenerated around the electrodes. As another processing method, the neurons stretching between electrodes were selectively cut by the direct femtosecond laser irradiation and the spontaneous electrical activity of the neuronal network was evaluated. While the spontaneous action potentials of neurons were synchronized before the cutting, the synchronization disappeared after the cutting, indicating that the neuronal network is locally disconnected by the laser cutting. The present method is applicable to artificial reconstruction of living neuronal network.

PACS

42.62.Be 87.19.Lj 52.38.Mf 

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Chie Hosokawa
    • 1
    • 2
  • Suguru N. Kudoh
    • 1
  • Ai Kiyohara
    • 1
    • 3
  • Yoichiroh Hosokawa
    • 4
  • Kazunori Okano
    • 4
  • Hiroshi Masuhara
    • 4
  • Takahisa Taguchi
    • 1
    • 3
  1. 1.Research Institute for Cell EngineeringNational Institute of Advanced Industrial Science and Technology (AIST)OsakaJapan
  2. 2.PRESTOJapan Science and Technology AgencySaitamaJapan
  3. 3.Graduate School of ScienceOsaka UniversityOsakaJapan
  4. 4.Hamano Life Science Research FoundationKobeJapan

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