Applied Physics A

, Volume 79, Issue 4–6, pp 795–798 | Cite as

Nondestructive isolation of single cultured animal cells by femtosecond laser-induced shockwave

  • Y. Hosokawa
  • H. Takabayashi
  • S. Miura
  • C. Shukunami
  • Y. Hiraki
  • H. Masuhara


A shockwave induced by focusing an intense femtosecond laser onto a culture medium under a microscope was used to manipulate single cultured animal cells. Mouse NIH3T3 fibroblasts were cultured on a collagen matrix and placed on a microscope installation that included an intense femtosecond laser. Cells were detached individually from the matrix by the shockwave, after their filopodia were cut by focusing the laser directly on them. The appearance and locomotion of cells after detachment was monitored with a CCD camera. Filopodia of the detached cells were regenerated, and the cell adhered again to the matrix within 4 hrs after detachment. When the shockwave was induced at a pulse energy of 0.72 μJ/pulse, 80% of cells were successfully detached from the culture plate in a non-destructive manner. The force required to detach a cell was estimated to be a few μN/μm2, which is larger than the photon force resulting from conventional laser trapping.


Collagen Shockwave Culture Plate Pulse Energy Femtosecond Laser 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Y. Hosokawa, M. Yashiro, T. Asahi, H. Masuhara: Appl. Surf. Sci. 154, 192 (2000)ADSCrossRefGoogle Scholar
  2. 2.
    K. Hatanaka, T. Itoh, T. Asahi, N. Ichinose, S. Kawanishi, T. Sasuga, H. Fukumura, H. Masuhara: Appl. Phys. Lett. 73, 3498 (1998)ADSCrossRefGoogle Scholar
  3. 3.
    Y. Hosokawa, M. Yashiro, T. Asahi, H. Masuhara, T. Kadota, Y. Shirota: Jap. J. Appl. Phys. 40, L116 (2001)Google Scholar
  4. 4.
    Y. Hosokawa, M. Yashiro, T. Asahi, H. Masuhara: Proc. SPIE: 4274, 78 (2001)ADSCrossRefGoogle Scholar
  5. 5.
    C.B. Schaffer, N. Nishimura, E.N. Glezer, A.M.-T. Kim, E. Mazur: Opt. Exp. 10, 196 (2002)ADSCrossRefGoogle Scholar
  6. 6.
    A. Vogel, J. Noack, K. Nahen, D. Theisen, S. Busch, U. Parlitz, D.X. Hammer, G.D. Noojin, B.A. Rockwell, R. Birngruber: Appl. Phys. B 68, 271 (1999)ADSCrossRefGoogle Scholar
  7. 7.
    J. Noack, D.X. Hammer, G.D. Noojin, B.A. Rockwell, A. Vogel: J. Appl. Phys. 83, 7488 (1998)ADSCrossRefGoogle Scholar
  8. 8.
    M.D. Feit, A.M. Rubenchik, B.M. Kim, L.B. da Silva, M.D. Perry: Appl. Surf. Sci. 127129,869 (1998)Google Scholar
  9. 9.
    T. Juhasz, R. Kurtz, C. Horvath, C. Suarez, F. Raksi, G. Spooner: Opt. Photonics News 13, 24 (2002)ADSCrossRefGoogle Scholar
  10. 10.
    F.H. Loesel, J.P. Fischer, M.H. Götz, C. Horvath, T. Juhasz, F. Noack, N. Suhm, J.F. Bille: Appl. Phys. B 66, 121 (1998)ADSGoogle Scholar
  11. 11.
    J. Krüger, W. Kautek, H. Newesely: Appl. Phys. A 69, S403 (1999)Google Scholar
  12. 12.
    J. Serbin, T. Bauer, C. Fallnich, A. Kasenbacher, W.H. Arnold: Appl. Surf. Sci. 197198, 737 (2002)Google Scholar
  13. 13.
    M.W. Berns, J. Aist, J. Edwards, K. Strahs, J. Girton, P. McNeill, J.B. Rattner, M. Kitzes, M. Hammer-Wilson, L.H. Liaw, A. Siemens, M. Koonce, S. Peterson, S. Brenner, J. Burt, R. Walter, P.J. Bryant, D. van Dyk, J. Coulombe, T. Cahill, G.S. Berns: Sci. 213, 505 (1981)ADSCrossRefGoogle Scholar
  14. 14.
    M.S. Hutson, Y. Tokutake, M.-S. Chang. J.W. Bloor, S. Venakides, D.P. Kiehart, G.S. Edwards: Sci. 300, 145 (2003)ADSCrossRefGoogle Scholar
  15. 15.
    F. Hoffmann: Plant Sci. 113, 1 (1996)CrossRefGoogle Scholar
  16. 16.
    N. Smith, K. Fujita, O. Nakamura, S. Kawata: Appl. Phys. Lett. 78, 999 (2001)ADSCrossRefGoogle Scholar
  17. 17.
    N. Smith, K. Fujita, T. Kaneko, K. Katoh, O. Nakamura, S. Kawata, T. Takamatsu: Appl. Phys. Lett. 79, 1208 (2001)ADSCrossRefGoogle Scholar
  18. 18.
    Y. Hosokawa, H. Masuhara, Y. Matsumoto, S. Sato: Proc. SPIE 4622, 138 (2002)ADSCrossRefGoogle Scholar
  19. 19.
    Y. Hosokawa, J. Takabayashi, H. Masuhara, K. Fujiyama, T. Seki, Y. Matsumoto, S. Sato: Int. Conf. LAMP 2002, 27, May (2002)Google Scholar
  20. 20.
    Y. Hosokawa, J. Takabayashi, Shukunami, Hiraki: Rev. Laser Eng. 32, 94 (2004)CrossRefGoogle Scholar
  21. 21.
    H. Misawa, M. Koshioka, K. Sasaki, N. Kitamura, H. Masuhara: J. Appl. Phys. 70, 3829 (1991)ADSCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Y. Hosokawa
    • 1
    • 3
  • H. Takabayashi
    • 1
    • 3
  • S. Miura
    • 2
  • C. Shukunami
    • 2
    • 3
  • Y. Hiraki
    • 2
    • 3
  • H. Masuhara
    • 1
    • 3
  1. 1.Dept. of Appl. Phys., Frontier Research Center and Venture Business Lab.Osaka Univ.OsakaJapan
  2. 2.Dept. of Cell. Differentiation, Inst. for Frontier Medical SciencesKyoto Univ.KyotoJapan
  3. 3.CREST-JSTSaitamaJapan

Personalised recommendations