Coral Reefs

, Volume 36, Issue 3, pp 773–784 | Cite as

Prevalent and persistent viral infection in cultures of the coral algal endosymbiont Symbiodinium

  • Karen D. Weynberg
  • Matthew Neave
  • Peta L. Clode
  • Christian R. Voolstra
  • Christopher Brownlee
  • Patrick Laffy
  • Nicole S. Webster
  • Rachel A. Levin
  • Elisha M. Wood-Charlson
  • Madeleine J. H. van Oppen
Report

Abstract

Reef corals are under threat from bleaching and disease outbreaks that target both the host animal and the algal symbionts within the coral holobiont. A viral origin for coral bleaching has been hypothesized, but direct evidence has remained elusive. Using a multifaceted approach incorporating flow cytometry, transmission electron microscopy, DNA and RNA virome sequencing, we show that type C1 Symbiodinium cultures host a nucleocytoplasmic large double-stranded DNA virus (NCLDV) related to Phycodnaviridae and Mimiviridae, a novel filamentous virus of unknown phylogenetic affiliation, and a single-stranded RNA virus related to retroviruses. We discuss implications of these findings for laboratory-based experiments using Symbiodinium cultures.

Keywords

Viruses Symbiodinium Symbiosis Dinoflagellate cultures Coral bleaching Scleractinia 

Notes

Acknowledgements

We acknowledge the technical support from Victor Beltran at the AIMS Symbiont Culture Facility and the scientific and technical assistance of the Australian Microscopy and Microanalysis Research Facility (AMMRF) at the Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, a facility funded by the University and the Western Australian and Australian governments. We acknowledge funding from the Australian Research Council SuperScience Fellowship #FS110200034 to K.D.W. and Future Fellowships #FT100100088 and #FT120100480 to M.v.O and N.S.W., respectively. Research reported in this publication was supported by King Abdullah University of Science and Technology.

Supplementary material

338_2017_1568_MOESM1_ESM.docx (28 kb)
Supplementary material 1 (DOCX 27 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Karen D. Weynberg
    • 1
  • Matthew Neave
    • 2
  • Peta L. Clode
    • 3
    • 4
  • Christian R. Voolstra
    • 2
  • Christopher Brownlee
    • 5
  • Patrick Laffy
    • 1
  • Nicole S. Webster
    • 1
  • Rachel A. Levin
    • 6
    • 7
  • Elisha M. Wood-Charlson
    • 1
    • 9
  • Madeleine J. H. van Oppen
    • 1
    • 8
  1. 1.Australian Institute of Marine ScienceTownsvilleAustralia
  2. 2.Division of Biological and Environmental Science and Engineering, Red Sea Research CenterKing Abdullah University of Science and TechnologyJeddahSaudi Arabia
  3. 3.Centre for Microscopy, Characterisation and AnalysisThe University of Western AustraliaPerthAustralia
  4. 4.The Oceans InstituteThe University of Western AustraliaPerthAustralia
  5. 5.Biological Resources Imaging LaboratoryUniversity of New South WalesSydneyAustralia
  6. 6.Centre for Marine Bio-InnovationThe University of New South WalesSydneyAustralia
  7. 7.Plant Functional Biology and Climate Change ClusterUniversity of Technology SydneySydneyAustralia
  8. 8.School of BioSciencesThe University of MelbourneParkville, MelbourneAustralia
  9. 9.Center for Microbial Oceanography: Research and EducationUniversity of Hawai‘i at MānoaHonoluluUSA

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