Advertisement

Mammalian Genome

, Volume 10, Issue 12, pp 1132–1136 | Cite as

The Belt mutation in pigs is an allele at the Dominant white (I/KIT) locus

  • Elisabetta  Giuffra
  • Gary  Evans
  • Anna  Törnsten
  • Richard  Wales
  • Andy  Day
  • Holger  Looft
  • Graham  Plastow
  • Leif  Andersson
Article

Abstract.

A white belt is a common coat color phenotype in pigs and is determined by a dominant allele (Be). Here we present the result of a genome scan performed using a Hampshire (Belt)/Pietrain (non-Belt) backcross segregating for the white belt trait. We demonstrate that Belt maps to the centromeric region of pig Chromosome (Chr) 8 harboring the Dominant white (I/KIT) locus. Complete cosegregation between Belt and a single nucleotide polymorphism in the KIT gene was observed. Another potential candidate gene, the endothelin receptor type A gene (EDNRA), was excluded as it was assigned to a different region (SSC8q21) by FISH analysis. We argue that Belt is a regulatory KIT mutation on the basis of comparative data on mouse KIT mutants and our previous sequence analysis of the KIT coding sequence from a Hampshire pig. Quantitative PCR analysis revealed that Belt is not associated with a KIT duplication, as is the case for the Patch and Dominant white alleles. Thus, Belt is a fourth allele at the Dominant white locus, and we suggest that it is denoted I Be .

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Elisabetta  Giuffra
    • 1
  • Gary  Evans
    • 2
  • Anna  Törnsten
    • 1
  • Richard  Wales
    • 2
  • Andy  Day
    • 2
  • Holger  Looft
    • 3
  • Graham  Plastow
    • 2
  • Leif  Andersson
    • 1
  1. 1.Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala Biomedical Centre, Box 597, S-751 24 Uppsala, SwedenSE
  2. 2.Molecular Biology Group, Pig Improvement Company, Cambridge CB1 2JN, UKGB
  3. 3.Deutsche PIG Improvement GmbH, Ratsteich 31, D-24837 Schleswig, GermanyDE

Personalised recommendations