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Mammalian Genome

, Volume 10, Issue 3, pp 271–276 | Cite as

Comparative mapping of 18 equine type I genes assigned by somatic cell hybrid analysis

  • Alexandre R.  Caetano
  • Daniel  Pomp
  • James D.  Murray
  • Ann T.  Bowling

Abstract.

Polymerase chain reaction primers designed from horse cDNA sequences and from consensus sequences highly conserved in mammalian species were used to amplify markers for synteny mapping 18 equine type I genes. These markers were used to screen a horse–mouse somatic cell hybrid panel (UCDavis SCH). Fourteen primer sets amplified horse-specific fragments, while restriction enzyme digests of PCR products were used to distinguish the fragments amplified from horse and mouse with four primer sets. Synteny assignments were made based on correlation values between each marker tested and other markers in the UCDavis SCH panel database. The 18 horse genes were assigned to previously established synteny groups. Synteny mapping of two genes previously mapped in the horse by FISH was used to anchor two UCD synteny groups to horse chromosomes. Previous chromosome assignments of three equine loci by FISH were confirmed. Comparative mapping analysis based on published human–horse Zoo-FISH data and the synteny mapping of 14 horse genes confirmed the physical assignment of 12 synteny groups to the respective horse chromosomes and was used to infer the physical location of one synteny group.

Keywords

Comparative Mapping Polymerase Chain Reaction Primer Restriction Enzyme Digest Somatic Cell Hybrid Chromosome Assignment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Alexandre R.  Caetano
    • 1
  • Daniel  Pomp
    • 2
  • James D.  Murray
    • 3
  • Ann T.  Bowling
    • 1
  1. 1.University of California-Davis, Veterinary Genetics Laboratory, Davis, California 95616-8744, USAUS
  2. 2.University of Nebraska-Lincoln, Department of Animal Science, Lincoln, Nebraska 68583-0908, USAUS
  3. 3.University of California-Davis, Animal Science Department, Davis, California 95616-8744, USAUS

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