Mammalian Genome

, Volume 9, Issue 12, pp 998–1001

Nonpermissiveness for mouse embryonic stem (ES) cell derivation circumvented by a single backcross to 129/Sv strain: establishment of ES cell lines bearing the Omd conditional lethal mutation

  • Chantal  Kress
  • Sandrine  Vandormael-Pournin
  • Patricia  Baldacci
  • Michel  Cohen-Tannoudji
  • Charles  Babinet

Abstract.

The inbred mouse strain DDK carries a conditional early embryonic lethal mutation that is manifested when DDK females are crossed to males of other inbred strains but not in the corresponding reciprocal crosses. It has been shown that embryonic lethality could be assigned to a single genetic locus called Ovum mutant (Om), on Chromosome (Chr) 11 near Syca 1. In the course of our study of the molecular mechanisms underlying the embryonic lethality, we were interested in deriving an embryonic stem cell bearing the Om mutation in the homozygous state (Omd/Omd). However, it turned out that DDK is nonpermissive for ES cell establishment, with a standard protocol. Here we show that permissiveness could be obtained using Omd/Omd blastocysts with a 75% 129/Sv and 25% DDK genetic background. Several germline-competent Omd/Omd ES cell lines have been derived from blastocysts of this genotype. Such a scenario could be extended to the generation of ES cell lines bearing any mutation present in an otherwise nonpermissive mouse strain.

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Copyright information

© Springer-Verlag New York Inc. 1998

Authors and Affiliations

  • Chantal  Kress
    • 1
  • Sandrine  Vandormael-Pournin
    • 1
  • Patricia  Baldacci
    • 1
  • Michel  Cohen-Tannoudji
    • 1
  • Charles  Babinet
    • 1
  1. 1.Unité de Biologie du Développement, URA C.N.R.S. 1960, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, FranceFR

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