Mammalian Genome

, Volume 9, Issue 6, pp 453–457

Mouse beta defensin-1 is a functional homolog of human beta defensin-1

  • Gillian M.  Morrison
  • Donald J.  Davidson
  • Fiona M.  Kilanowski
  • Duncan W.  Borthwick
  • Ken  Crook
  • Alison I.  Maxwell
  • John R.W.  Govan
  • Julia R.  Dorin

DOI: 10.1007/s003359900795

Cite this article as:
Morrison, G., Davidson, D., Kilanowski, F. et al. (1998) 9: 453. doi:10.1007/s003359900795

Abstract.

Defensin are 3–4 kDa antimicrobial peptides of which three distinct families have been identified; α-defensin, β-defensins, and insect defensins. Recent investigations have shown that β-defensins are present in the human airways and may be relevant to the pathogenesis of cystic fibrosis (CF) lung disease. We report here the further characterization of a recently identified mouse β-defensin gene, Defb1, sometimes referred to as mBD-1, which is homologous to the human airway beta defensin hBD-1. We report that Defb1 is expressed in a variety of tissues including the airways and, similar to hBD-1, is not upregulated by lipopolysaccharide (LPS). Defb1 was found to consist of two small exons separated by a 16-kb intron and cytogenetic, and physical mapping linked it to the alpha defensin gene cluster on mouse Chromosome (Chr) 8. Functional studies demonstrate that, like hBD-1, Defb1 demonstrates a salt-sensitive antimicrobial activity against Pseudomonas aeruginosa. Of relevance to CF lung disease is the fact that neither the hBD-1 nor the mBD-1 peptides are active against Burkholderia cepacia.

Copyright information

© Springer-Verlag New York Inc. 1998

Authors and Affiliations

  • Gillian M.  Morrison
    • 1
  • Donald J.  Davidson
    • 1
  • Fiona M.  Kilanowski
    • 1
  • Duncan W.  Borthwick
    • 1
  • Ken  Crook
    • 1
  • Alison I.  Maxwell
    • 2
  • John R.W.  Govan
    • 2
  • Julia R.  Dorin
    • 1
  1. 1.MRC Human Genetics Unit, Western General Hospital, Edinburgh, EH4 2XU, Scotland, UKGB
  2. 2.Department of Medical Microbiology, University of Edinburgh Medical School, Teviot Place, Edinburgh, EH8 9AG, Scotland, UKGB

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