Mammalian Genome

, Volume 9, Issue 3, pp 210–213

Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle

  • Luc Grobet
  • Dominique Poncelet
  • Luis José Royo
  • Benoit Brouwers
  • Dimitri Pirottin
  • Charles Michaux
  • François Ménissier
  • Marta Zanotti
  • Susana Dunner
  • Michel Georges
Original Contributions

DOI: 10.1007/s003359900727

Cite this article as:
Grobet, L., Poncelet, D., Royo, L.J. et al. Mammalian Genome (1998) 9: 210. doi:10.1007/s003359900727

Abstract

We have determined the entire myostatin coding sequence for 32 double-muscled cattle sampled from ten European cattle breeds. Seven DNA sequence polymorphisms were identified, of which five would be predicted to disrupt the function of the protein, one is a conservative amino acid substitution, and one a silent DNA sequence variant. Four additional DNA sequence polymorphisms were identified in myostatin intronic sequences. In all but two breeds, all double-muscled animals were either homozygous or compound heterozygotes for one of the five loss-offunction mutations. The absence of obvious loss-of-function mutations in the coding sequence of the two remaining breeds points either towards additional mutations in unexplored segments of the gene, or towards locus heterogeneity of double-muscling.

Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • Luc Grobet
    • 1
  • Dominique Poncelet
    • 1
  • Luis José Royo
    • 2
  • Benoit Brouwers
    • 1
  • Dimitri Pirottin
    • 1
  • Charles Michaux
    • 1
  • François Ménissier
    • 3
  • Marta Zanotti
    • 4
  • Susana Dunner
    • 2
  • Michel Georges
    • 1
  1. 1.Department of Genetics, Faculty of Veterinary MedicineUniversity of Liège (B43)LiègeBelgium
  2. 2.Laboratorio de Genetica, Dpto. de Produccion AnimalUniversidad ComplutenseMadridSpain
  3. 3.Station de Génétique Quantitative et AppliquéeI. N. R. A.Jouy-en-JosasFrance
  4. 4.Istituto di Zootecnia, facolta di Medicina VeterinariaUniversita degli Studi di MilanoMilanoItaly

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