Interval-specific congenic strains (ISCS): An experimental design for mapping a QTL into a 1-centimorgan interval
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A general experimental design that allows mapping of a quantitative trait locus (QTL) into a 1-cM interval is presented. The design consists of a series of strains, termed “interval-specific congenic strains (ISCS)”. Each ISCS is recombinant at a specific 1-cM sub-interval out of an ordered set of sub-intervals, which together comprise a wider interval, to which a QTL was previously mapped. It is shown that a specific and previously detected QTL of moderate or even small effect can be accurately mapped into a 1-cM interval in a program involving a total of no more than 1000 individuals. Consequently, ISCS can serve as the ultimate genetic mapping procedure before the application of physical mapping tools for positional cloning of a QTL.
KeywordsQuantitative Trait Locus Congenic Strain Quantitative Trait Locus Location Quantitative Trait Locus Genotype Congenic Mouse Strain
- Changjian J, Zeng ZB (1995) Multiple trait analysis of genetic mapping for quantitative trait loci. Genetics 140, 1111–1127Google Scholar
- Darvasi A, Soller M (1997) A simple method to calculate resolving power and confidence interval of QTL map location. Behavior Genetics (in press)Google Scholar
- Hilbert P, Lindpainter K, Beckmann JS, Serikawa T, Soubrier F, Dubay C, Cartwright P, De Gouyon B, Julier C, Takahashi S, Vincent M, Ganten D, Georges M, Lathrop GM (1991) Chromosomal mapping of two genetic loci associated with blood pressure regulation in hereditary hypertensive rats. Nature 353, 521–529PubMedCrossRefGoogle Scholar
- Nienhuis J, Helentjaris T, Slocum M, Ruggero B, Schaeffer A (1987). Restriction fragment length polymorphism analysis of loci associated with insect resistance in tomato. Crop Sci 27, 797–803Google Scholar