Mammalian Genome

, Volume 8, Issue 2, pp 129–133 | Cite as

CAND3: A ubiquitously expressed gene immediately adjacent and in opposite transcriptional orientation to the ATM gene at 1lq23.1

  • Xiaeguang Chen
  • Lan Yang
  • Nitin Udar
  • Teresa Liang
  • Nancy Uhrhammer
  • Shunbin Xu
  • Jacques -Olivier Bay
  • Zhijun Wang
  • Suganda Dandakar
  • Sujata Chiplunkar
  • Ivana Klisak
  • Milhan Telatar
  • Huanmin Yang
  • Patrick Concannon
  • Richard A. Gatti
Original Contribution

Abstract

Using a magnetic beads-mediated cDNA selection procedure and a fetal brain expression library, we identified a transcriptional unit within a cosmid positive for the marker D11S384. Pursuit of its full-length cDNA led to the cloning of the third candidate gene (CAND3) we studied in our quest for the ataxiatelangiectasia (A-T) gene, ATM. CAND3 spans ~140 kb of genomic DNA and is located immediately centrimeric to ATM, with 544 bp of DNA separating the two genes. CAND3 encodes two ubiquitously expressed transcripts of ~5.8 kb and ~4.6 kb that are divergently transcribed from a promoter region common to ATM. Nucleotide sequence was determined for one of its alternately spliced transcripts. The predicted protein has 1175 amino acids and is novel in sequence, with only weak homologies to transcriptional factors, nucleoporin protein, and protein kinases, including members of the phosphatidylinositol 3-kinase (PI-3 kinase) family. Although neither homology to ATM nor any mutation of CAND3 in A-T patients has been found, the head-to-head arrangement of CAND3 and ATM, with expression of both housekeeping genes from a common stretch of 544 bp intergenic DNA, suggests a bi-directional promoter possibly for co-regulation of biologically related functions. YACs, BACs, cosmids, and STSs are defined to aid in the further study of this gene.

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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Xiaeguang Chen
    • 1
  • Lan Yang
    • 1
  • Nitin Udar
    • 1
  • Teresa Liang
    • 1
  • Nancy Uhrhammer
    • 1
  • Shunbin Xu
    • 1
  • Jacques -Olivier Bay
    • 1
  • Zhijun Wang
    • 1
  • Suganda Dandakar
    • 1
  • Sujata Chiplunkar
    • 1
  • Ivana Klisak
    • 1
  • Milhan Telatar
    • 1
  • Huanmin Yang
    • 1
  • Patrick Concannon
    • 2
  • Richard A. Gatti
    • 1
  1. 1.Department of PathologyUCLA School of MedicineLos AngelesUSA
  2. 2.Virginia Mason Research Center and the Department of ImmunologyUniversity of Washington School of MedicineSeattleUSA

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