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Mammalian Genome

, Volume 8, Issue 2, pp 121–128 | Cite as

Linkage map of Syrian hamster with restriction landmark genomic scanning

  • H. Okuizumi
  • T. Ohsumi
  • N. Sasaki
  • H. Imoto
  • Y. Mizuno
  • T. Hanami
  • H. Yamashita
  • M. Kamiya
  • S. Takada
  • A. Kitamura
  • M. Muramatsu
  • M. Nishimura
  • M. Mori
  • Y. Matsuda
  • O. Tagaya
  • Y. Okazaki
  • Y. Hayashizaki
Original Contribution

Abstract

We have constructed the linkage map with precise genetic analysis of the Syrian hamster, Mesocricetus auratus, according to the restriction landmark genomic scanning (RLGS) spot mapping method. Although only 3.2–6.6% of the total RLGS spots between the two strains, ACN and BIO 14.6, showed genetic variance, 572 loci were found to be polymorphic. Out of 569 RLGS loci and 3 other loci, 531 were mapped with the backcross (ACN x BIO 14.6) F1 x BIO 14.6. The cumulative map was 1111.6 cM, indicating that the spots/loci are located throughout the genome at 1.94 cM intervals on average. Thus, RLGS provides us with a rapid tool to construct the genetic map of any species, even if it has less genetic variation.

Keywords

Linkage Group Syrian Hamster Backcross Progeny Simple Sequence Length Polymorphism Restriction Landmark Genomic Scanning 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • H. Okuizumi
    • 1
    • 2
  • T. Ohsumi
    • 1
  • N. Sasaki
    • 1
  • H. Imoto
    • 1
  • Y. Mizuno
    • 1
  • T. Hanami
    • 1
  • H. Yamashita
    • 1
  • M. Kamiya
    • 1
  • S. Takada
    • 1
  • A. Kitamura
    • 1
  • M. Muramatsu
    • 1
  • M. Nishimura
    • 3
  • M. Mori
    • 3
  • Y. Matsuda
    • 4
  • O. Tagaya
    • 5
  • Y. Okazaki
    • 1
  • Y. Hayashizaki
    • 1
  1. 1.Genome Science LaboratoryTsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN)Tsukuba, IbarakiJapan
  2. 2.Ecological Genetics LaboratoryForestry and Forest Products Research InstituteTsukuba, IbarakiJapan
  3. 3.Institute for Experimental AnimalsHamamatsu University School of MedicineHamamatsuJapan
  4. 4.Animal Gene Control Laboratory, School of Agricultural SciencesNagoya UniversityChigusa, NagoyaJapan
  5. 5.Department of Planning & ResourcesResearch Division, Eisai Co., Ltd.Bunkyo-ku, TokyoJapan

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