Mammalian Genome

, Volume 7, Issue 7, pp 481–485

Exclusion of Sox9 as a candidate for the mouse mutant Tail-short

  • K. Uchida
  • P. Koopman
  • A. Mita
  • S. Wakana
  • E. Wright
  • Y. Kikkawa
  • H. Yonekawa
  • K. Moriwaki
  • T. Shiroishi
Original Contribution

DOI: 10.1007/s003359900147

Cite this article as:
Uchida, K., Koopman, P., Mita, A. et al. Mammalian Genome (1996) 7: 481. doi:10.1007/s003359900147

Abstract

The Sry-related gene Sox9 has been proposed as the gene responsible for the mouse skeletal mutant Tail-short (Ts), on the basis of its expression in skeletogenic mesenchymal condensations in the mouse embryo and its chromosomal location in the region of Ts on distal Chromosome (Chr) 11. We present here detailed mapping of Ts locus relative to the Sox9, using an inter-subspecific cross. Among 521 backcross progeny, 16 recombinants were detected between Sox9 and Ts, suggesting a separation of 3.5 ± 0.01 cM, and excluding Sox9 as a candidate for Ts. A further nine recombinants were detected between Ts and the poly-comb-like gene M33, suggesting that these loci are separated by 1.8 ± 0.011 cM. Six microsatellite markers were co-localized to the Ts locus, providing reagents for positional cloning of Ts.

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • K. Uchida
    • 1
  • P. Koopman
    • 2
    • 3
  • A. Mita
    • 1
  • S. Wakana
    • 4
  • E. Wright
    • 2
  • Y. Kikkawa
    • 5
  • H. Yonekawa
    • 5
  • K. Moriwaki
    • 6
  • T. Shiroishi
    • 1
  1. 1.Mammalian Genetics LaboratoryNational Institute of GeneticsYataJapan
  2. 2.Centre for Molecular and Cellular BiologyThe University of QueenslandBrisbaneAustralia
  3. 3.Department of Anatomical SciencesThe University of QueenslandBrisbaneAustralia
  4. 4.Central Institute for Experimental AnimalsMiyamae-kuJapan
  5. 5.Department of Laboratory Animal ScienceThe Tokyo Metropolitan Institute of Medical ScienceHonkomagome, TokyoJapan
  6. 6.The Graduate University for Advanced StudiesHayama, Kanagawa-kenJapan

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