Mammalian Genome

, Volume 7, Issue 5, pp 340–343 | Cite as

Generation and mapping of Mus spretus strain-specific markers for rapid genomic scanning

Original Contribution
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Accepted:

Abstract

We describe here a set of genetic markers, based on IRS-PCR amplification difference, that are specifically designed for efficient, high throughput genetic mapping in [(M. domesticus × wild-derived) F1 × M. domesticus] interspecific backcrosses. 146 new genetic loci have been mapped, and strain distribution for these markers has been determined in 96 mouse strains. 103 (81%) of 127 tested markers are present only in one or more wild-derived strains, but absent in 76 other commonly used strains, demonstrating their utility in a variety of mouse pair combinations. Because of the ease of genotyping with this marker set, rapid genome scans for complex genetic trait loci involving crosses between wild-derived strains and other commonly used strains can now be carried out efficiently with large numbers of animals.

Keywords

Mouse Genome Database Interspecific Backcross Cation Difference Backcross Panel Yeast Artificial Chromosome Contigs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  1. 1.Center for Cancer ResearchMassachusetts Institute of TechnologyCambridgeUSA

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