Mammalian Genome

, Volume 7, Issue 4, pp 280–284 | Cite as

Construction of a cytogenetically characterized porcine somatic cell hybrid panel and its use as a mapping tool

  • C. Zijlstra
  • A. A. Bosma
  • N. A. de Haan
  • C. Mellink
Original Contributions
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Accepted:

Abstract

A new panel of cytogenetically characterized pigrodent somatic cell hybrids was constructed and tested for twelve microsatellite markers with PCR. Cytogenetic characterization of hybrids was accomplished by fluorescence painting and GTG-banding of metaphase chromosomes. The panel consists of 15 independent pig-hamster and 6 independent pig-mouse cell lines. In the panel, all pig autosomes and the X Chromosome (Chr) are represented, and it is informative for all chromosome pairs except 2–14, 2–15, 3–9, 14–15, 14–16, and 16–17. The microsatellites tested were S0022, S0023, S0084, S0098, SO112, SO113, SO114, S0115, S0117, S0118, S0119, and S0120. The PCR results obtained in the 21 hybrids were compared with the cytogenetic data and analyzed for concordancy and correlation. Eight microsatellites could be assigned to specific pig chromosomes, confirming seven assignments based on linkage analysis.

Keywords

Chromosome Painting Somatic Cell Hybrid Hybrid Cell Line Chromosomal Assignment Rodent Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • C. Zijlstra
    • 1
  • A. A. Bosma
    • 1
  • N. A. de Haan
    • 1
  • C. Mellink
    • 1
  1. 1.Department of Cell Biology and Histology, Faculty of Veterinary MedicineUtrecht UniversityTD UtrechtThe Netherlands

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