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Mammalian Genome

, Volume 7, Issue 1, pp 55–58 | Cite as

Genetic composition of the recombinant congenic strains

  • A. P. M. Stassen
  • P. C. Groot
  • J. T. Eppig
  • P. Demant
Original Contributions

Abstract

For the study of biological phenomena influenced by multiple genes in mice, the Recombinant Congenic Strains (RCS) have been developed. An RCS series comprises approximately 20 homozygous strains, each of which contains on average 87.5% genes of a common background strain and 12.5% of a common donor strain. In an RCS series, non-linked genes involved in the control of a multigenic trait become distributed into different re-combinant congenic strains. In this way a multigenic trait is transformed into a series of single gene traits in which each gene can be studied individually. For the ability to use the strength of the recombinant congenic strains system to its full extent, a thorough genetic characterization is indispensable. We have typed the CcS/ Dem and OcB/Dem series for 611 and 550 markers, respectively. This results in a genetic characterization sufficient to detect most donor strain genes. In addition, we report the genetic characterization of the HcB/Dem and HcB(N4)/Dem series. Strains of the latter series contain on average 6.25% of the donor strain genome. Both series have been typed for 130 markers. All the typing data have been deposited in the Mouse Genome Database at The Jackson Laboratory.

Keywords

Simple Sequence Repeat Marker Genetic Characterization Genetic Composition Background Strain Donor Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • A. P. M. Stassen
    • 1
  • P. C. Groot
    • 1
  • J. T. Eppig
    • 2
  • P. Demant
    • 1
  1. 1.Division of Molecular GeneticsThe Netherlands Cancer InstituteCX AmsterdamThe Netherlands
  2. 2.Mouse Genome InformaticsThe Jackson LaboratoryBar HarborUSA

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