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Mammalian Genome

, Volume 12, Issue 5, pp 376–379 | Cite as

The PrP-like protein Doppel gene in sheep and cattle: cDNA sequence and expression

  • Michael A.  Tranulis
  • Arild  Espenes
  • Sergio  Comincini
  • Grethe  Skretting
  • Ingrid  Harbitz

Abstract.

cDNAs encoding the ovine and bovine prion protein-like protein Doppel (Dpl) have been cloned. Sequencing revealed cDNAs of 2.85 and 3.31 kb from ovine and bovine testicular tissue, in accordance with observations of single transcripts of 3.2 and 3.6 kb on Northern blots. Sequence alignments showed a very high degree of identity between the sheep and cattle Dpl cDNAs, except for a 0.4-kb stretch in the bovine 3′ untranslated region and the terminal 3′ end of the sequences. The expression pattern of the Dpl gene (Prnd) in adult tissues from both species was compared by Northern blot and RT-PCR analyses. The Prnd gene was expressed strongly in testicular tissue, while low levels of expression were seen in other tissues. The open reading frame of the ovine and bovine sequences encodes a 178-amino acid protein with 95% sequence identity between the two species. Predicted structural features are in close agreement with previous reports for mouse, human, and rat Dpl.

Keywords

Structural Feature Sequence Alignment Northern Blot cDNA Sequence Untranslated Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Michael A.  Tranulis
    • 1
  • Arild  Espenes
    • 2
  • Sergio  Comincini
    • 3
  • Grethe  Skretting
    • 2
  • Ingrid  Harbitz
    • 1
  1. 1.Department of Biochemistry, Physiology and Nutrition, Norwegian School of Veterinary Science, N-0033 Oslo, NorwayNO
  2. 2.Department of Morphology, Genetics and Aquatic Biology, Norwegian School of Veterinary Science, N-0033 Oslo, NorwayNO
  3. 3.Dipartimento de Genetica e Microbiologia, Università di Pavia, via Abbiategrasso 207, 27100 Pavia, ItalyIT

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