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Mammalian Genome

, Volume 12, Issue 2, pp 129–132 | Cite as

Murine phosphatidylserine-specific phospholipase A1 (Ps-pla1) maps to Chromosome 16 but is distinct from the lpd (lipid defect) locus

  • Xiao-Yan  Wen
  • A. Keith  Stewart
  • Jennifer  Skaug
  • Ellen  Wei
  • Lap-Chee  Tsui

Abstract.

We have previously generated a mouse transgenic line with an insertional mutation designated lpd that demonstrates a phenotype of hypertriglyceridemia and fatty liver. Since the recently identified phosphatidylserine-specific phospholipase A1 (PS-PLA1) demonstrates significant homology to triglyceride lipases, we reasoned that the mouse Ps-pla1 gene may be the disrupted gene within the lpd locus. Using a rat PS-PLA1 cDNA sequence to search the EST database, we identified a mouse EST homolog AA839424. Sequencing analysis of AA839424 revealed a putative Ps-pla1 protein of 456 amino acids with extensive overall structural conservation with human and rat PS-PLA1 and with triglyceride lipases. Conserved sequences in Ps-pla1 include a lipase consensus sequences G×S×G, a catalytic triad, and eight of the ten conserved cysteine residues that are required for tertiary structure. Mouse Ps-pla1 carries a phosphatidylserine-binding motif that is absent in all triglyceride lipases. Using a mouse whole-genome radiation hybrid (WG-RH) mapping panel (T31), we mapped mouse Ps-pla1 to Chromosome (Chr) 16 between genetic markers D16Mit194 and D16Mit38, which is 17.1 cM centromeric to the lpd locus. On the basis of chromosome location, we conclude that Ps-pla1 and lpd are distinct genes in lipid metabolism.

Keywords

Lipase Transgenic Line Triad Fatty Liver Insertional Mutation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Xiao-Yan  Wen
    • 1
  • A. Keith  Stewart
    • 1
  • Jennifer  Skaug
    • 2
  • Ellen  Wei
    • 1
  • Lap-Chee  Tsui
    • 2
  1. 1.Department of Experimental Therapeutics, Toronto General Hospital Research Institute, 67 College Street CBS456, University Health Network, Toronto, Ontario, Canada M5G 2M1CA
  2. 2.Department of Genetics, The Hospital for Sick Children Research Institute, Toronto, Ontario, Canada M5G 1X8CA

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