Mammalian Genome

, Volume 12, Issue 3, pp 212–218 | Cite as

Canine homolog of the T-box transcription factor T; failure of the protein to bind to its DNA target leads to a short-tail phenotype

  • Kim  Haworth
  • Wendy  Putt
  • Bruce  Cattanach
  • Matthew  Breen
  • Matthew  Binns
  • Frode  Lingaas
  • Yvonne H.  Edwards

Abstract.

Domestic dog breeds show a wide variety of morphologies and offer excellent opportunities to study the molecular genetics of phenotypic traits. We are interested in exploring this potential and have begun by investigating the genetic basis of a short-tail trait. Our focus has been on the T gene, which encodes a T-box transcription factor important for normal posterior mesoderm development. Haploinsufficiency of T protein underlies a short-tail phenotype in mice that is inherited in an autosomal dominant fashion. We have cloned the dog homolog of T and mapped the locus to canine Chromosome (Chr) 1q23. Full sequence analysis of the T gene from a number of different dog breeds identified several polymorphisms and a unique missense mutation in a bob-tailed dog and its bob-tailed descendants. This mutation is situated in a highly conserved region of the T-box domain and alters the ability of the T protein to bind to its consensus DNA target. Analysis of offspring from several independent bobtail × bobtail crosses indicates that the homozygous phenotype is embryonic lethal.

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Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Kim  Haworth
    • 1
  • Wendy  Putt
    • 1
  • Bruce  Cattanach
    • 2
  • Matthew  Breen
    • 3
  • Matthew  Binns
    • 3
  • Frode  Lingaas
    • 4
  • Yvonne H.  Edwards
    • 1
  1. 1.MRC Human Biochemical Genetics Unit, University College London, Wolfson House, 4 Stephenson Way, London NW1 2HE, UKGB
  2. 2.MRC Mammalian Genetics Unit, Harwell, Didcot, Oxfordshire, OX11 ORD, UKGB
  3. 3.Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk CB8 7UU, UKGB
  4. 4.Section of Genetics, Norwegian School of Veterinary Science PO Box 8146 Dep., N-0033 Oslo, NorwayNO

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