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Mammalian Genome

, Volume 12, Issue 1, pp 13–16 | Cite as

High-resolution genetic mapping of the saccharin preference locus (Sac) and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal Chromosome 4

  • Xia  Li
  • Masashi  Inoue
  • Danielle R.  Reed
  • Taufiqul  Huque
  • Ralph B.  Puchalski
  • Michael G.  Tordoff
  • Yuzo  Ninomiya
  • Gary K.  Beauchamp
  • Alexander A.  Bachmanov

Abstract.

The Sac (saccharin preference) locus affecting mouse behavioral and neural responsiveness to sweeteners has been mapped to distal Chr 4. A putative sweet taste receptor, T1R1, has been recently cloned, and the gene encoding it, Gpr70, has also been mapped to mouse distal Chr 4. To assess Gpr70 as a candidate gene for Sac, we compared the Gpr70 sequences of C57BL/6ByJ and 129P3/J mouse strains with different alleles of Sac. Using Gpr70 sequence variation between the C57BL/6ByJ and 129P3/J strains, we conducted a high-resolution analysis of the chromosomal localization of the Gpr70 and Sac loci in the F2 hybrids and 129.B6-Sac partially congenic mice originating from these two strains. The Gpr70 gene maps proximal to Sac, which demonstrates that they are different loci.

Keywords

Candidate Gene Sequence Variation Genetic Mapping Mouse Strain Chromosomal Localization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Xia  Li
    • 1
  • Masashi  Inoue
    • 2
  • Danielle R.  Reed
    • 1
  • Taufiqul  Huque
    • 1
  • Ralph B.  Puchalski
    • 1
  • Michael G.  Tordoff
    • 1
  • Yuzo  Ninomiya
    • 3
  • Gary K.  Beauchamp
    • 1
  • Alexander A.  Bachmanov
    • 1
  1. 1.Monell Chemical Senses Center, 3500 Market Street, Philadelphia, Pennsylvania 19104, USAUS
  2. 2.Laboratory of Cellular Neurobiology, School of Life Science, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-03, JapanJP
  3. 3.Department of Oral Physiology, Faculty of Dentistry, Kyushu University, 3-1-1, Maidashi, Higashi-Ku, Fukuoka 812-8582, JapanJP

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