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Mammalian Genome

, Volume 11, Issue 12, pp 1098–1106 | Cite as

Comparative mapping between humans and pigs: localization of 58 anchorage markers (TOASTs) by use of porcine somatic cell and radiation hybrid panels

  • Y.  Lahbib-Mansais
  • S.  Leroux
  • D.  Milan
  • M.  Yerle
  • A.  Robic
  • Z.  Jiang
  • C.  André
  • J.  Gellin

Abstract.

To increase the number of Type I markers that are directly informative for comparative mapping, 58 anchorage markers, TOASTs (Traced Orthologous Amplified Sequence Tags), were mapped in pig. With specific consensus primers, 76 TOASTs were tested in pig: 50 were regionally localized in pig on a somatic cell hybrid panel (SCHP), and 51 were mapped on the whole genome, INRA/University of Minnesota porcine Radiation Hybrid panel (IMpRH). Comparison of marker positions on RH and cytogenetic maps indicated general concordance except for two chromosomal regions. For RH mapping, all markers, apart from one, were significantly linked (LOD > 4.8) to a marker of the first-generation radiation hybrid map. Localization of new markers on the initial map is necessary for drawing a framework map as shown for Chromosome Sscr 14. The addition of four TOASTs has enabled us to propose an improved map, using a threshold likelihood ratio of 1000/1. At the whole-genome level, this work significantly increased (by 50%) the number of precisely mapped genes on the porcine RH map and confirmed that the IMpRH panel is a valuable tool for high-resolution gene mapping in pig. Porcine PCR products were sequenced and compared with human sequences to verify their identity. Most of the localizations made it possible to either confirm or refine the previous comparative data between humans and pigs obtained through heterologous chromosomal painting or gene mapping. Moreover, the use of TOASTs in mapping studies appears to be a complement to other strategies using CATS, human ESTs, or heterologous FISH with BACs which had already been applied to improve the gene density of comparative genomic maps for mammals.

Keywords

Radiation Hybrid Chromosomal Painting Anchorage Marker Radiation Hybrid Panel Somatic Cell Hybrid Panel 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • Y.  Lahbib-Mansais
    • 1
  • S.  Leroux
    • 1
  • D.  Milan
    • 1
  • M.  Yerle
    • 1
  • A.  Robic
    • 1
  • Z.  Jiang
    • 3
  • C.  André
    • 2
  • J.  Gellin
    • 1
  1. 1.Institut National de la Recherche Agronomique (INRA), BP27, 31326 Castanet-Tolosan Cedex, FranceFR
  2. 2.UPR41 CNRS, Faculté de Médecine, 2 avenue Professor Léon Bernard, 35043 Rennes Cedex, FranceFR
  3. 3.Centre for Genetic Improvement of Livestock, Department of Animal and Poultry Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1CA

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