Mammalian Genome

, Volume 11, Issue 7, pp 572–576

Cryopreservation of mouse spermatozoa

  • N.  Nakagata

DOI: 10.1007/s003350010109

Cite this article as:
Nakagata, N. Mammalian Genome (2000) 11: 572. doi:10.1007/s003350010109


Recently, it has become possible to freeze a large number of mouse spermatozoa immediately after collection from the epididymides of a small number of males. The cryopreservation of spermatozoa is simpler, less time-consuming, and less costly than that of embryos for maintaining various strains of mice with induced mutations. This chapter attempts to provide a realistic overview of the cryopreservation of mouse spermatozoa and to describe a detailed procedure for mouse sperm freezing.

Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • N.  Nakagata
    • 1
  1. 1.Division of Reproductive Engineering, Center for Animal Resources & Development, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, JapanJP

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