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Mammalian Genome

, Volume 20, Issue 9–10, pp 532–543 | Cite as

Imprinting and epigenetic changes in the early embryo

  • Jamie R. Weaver
  • Martha Susiarjo
  • Marisa S. Bartolomei
Article

Abstract

Imprinted genes are epigenetically regulated so that only one allele is expressed in a parent-of-origin-dependent manner. Although they represent a small subset of the mammalian genome, imprinted genes are essential for normal development. The regulatory mechanisms underlying imprinting are complex and have been the subject of extensive investigation. DNA methylation is the best-established epigenetic mark that is critical for the allele-specific expression of imprinted genes. This mark must be correctly established in the germline, maintained throughout life, and erased and reestablished in the germline the next generation. These events coincide with the genome-wide epigenetic reprogramming that occurs during gametogenesis and early embryogenesis; therefore, the establishment and maintenance of DNA methylation must be tightly regulated. Studies on enzymes that participate in both de novo methylation and its maintenance (i.e., the DNMT family) have provided information on how methylation influences imprinting. However, many aspects of the regulation of DNA methylation are unknown, including how methylation complexes are targeted and the molecular mechanisms underlying DNA demethylation. In this review we focus on the epigenetic changes that occur in the germline and early embryo, with an emphasis on imprinting. We summarize recent findings on factors influencing DNA methylation establishment, maintenance, and erasure that have further elucidated the mechanisms of imprinting, while highlighting topics that require further investigation.

Keywords

Histone Modification Imprint Gene Primordial Germ Cell Preimplantation Embryo Male Germ Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

JRW is supported by T32 GMO7229. The work in the authors’ laboratory has been supported by the Public Health Services grants GM51279, GM74768, and HD42026.

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© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jamie R. Weaver
    • 1
  • Martha Susiarjo
    • 1
  • Marisa S. Bartolomei
    • 1
  1. 1.Department of Cell and Developmental BiologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA

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