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Mammalian Genome

, Volume 20, Issue 7, pp 404–413 | Cite as

A novel ENU-induced mutation, peewee, causes dwarfism in the mouse

  • Lee Bokryeon
  • Kiyoshi Kano
  • Jay Young
  • Simon W. M. John
  • Patsy M. Nishina
  • Jurgen K. Naggert
  • Kunihiko Naito
Article
  • 155 Downloads

Abstract

We identified a novel fertile autosomal recessive mutation called peewee that results in dwarfing, in a region-specific ENU-induced mutagenesis. These mice at litter size were smaller those of other strains. Histological analysis revealed that the major organs appear normal, but abnormalities in cellular proliferation were observed in bone, liver, and testis. Haplotype analysis localized the peewee gene to a 3.3-Mb region between D5Mit83 and D5Mit356.3. There are 18 genes in this linkage area. We also performed in silico mapping using the PosMedSM program, which searches for connections among keywords and genes in an interval, but no similar phenotype descriptions were found for these genes. In the peewee mutant compared to the normal C57BL/6 J mouse, only Slc10a4 expression was lower. Our preliminary mutation analysis examining the nucleotide sequence of three exons, two introns, and an untranslated region of Slc10a4 did not find any sequence difference between the peewee mouse and the C57BL/6 J mouse. Detailed analysis of peewee mice might provide novel molecular insights into the complex mechanisms regulating body growth.

Keywords

Discoidin Domain Receptor Dwarf Mouse Endochondral Bone Formation Autosomal Recessive Mutation Deoxyuridine Triphosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Dr. Neena Haider and Dr. Arne Nystuen for their critiques of the manuscript. This research was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, the Morinaga Foundation, the Foundation for Growth Science, and the U.S. National Institutes of Health (grants DK46977 and DK73267).

Supplementary material

335_2009_9197_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 17 kb)
335_2009_9197_MOESM2_ESM.eps (759 kb)
Supplementary Fig. 1 The litter sizes of peewee mice and inbred line (EPS 759 kb)
335_2009_9197_MOESM3_ESM.eps (7 mb)
Supplementary Fig. 2 Cross sections of various tissues from peewee mutant, stained with hematoxylin and eosin, at 10 weeks of age. Brain (A), pituitary gland (B), eye (C), heart (D), lung (E), spleen (F), skin (G), skeletal muscle (H), pancreas (I), liver (J), stomach (K), intestine (L), kidney (M), uterus (N), ovary (O), and testis (P). Scale bar = 25 μm (C, D, E), 100 μm (F, G, H, J, K, L), 200 μm (A, B, C, M, N, O, P, Q) (EPS 7217 kb)
335_2009_9197_MOESM4_ESM.eps (810 kb)
Supplementary material 4 (EPS 810 kb)

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Lee Bokryeon
    • 1
  • Kiyoshi Kano
    • 1
  • Jay Young
    • 2
  • Simon W. M. John
    • 2
  • Patsy M. Nishina
    • 2
  • Jurgen K. Naggert
    • 2
  • Kunihiko Naito
    • 1
  1. 1.Laboratory of Applied Genetics, Graduate School of Agricultural and Life ScienceUniversity of TokyoTokyoJapan
  2. 2.The Jackson LaboratoryBar HarborUSA

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